Note:M.250 bp Marker;1.Control of pUC19-HB-T plasmid;2.pUC19-HB-T plasmid was digested with EcoR I;3.pUC19-HB-T plasmid was digested with BamH I;4.pUC19-HB-T plasmid was digested with Asc I;5.pUC19-HB-T plasmid was digested with Xcm I;6.pUC19-HB-T plasmid was digested with ...
[Method]Twocomple— mentaryoligonuckotidechainscontainingtwomIsitesweresynthesized.Atierdenaturation andrenaturation,theadaptorwasclonedinto plasmidpUC19betweentheHindIIIandBarnHIsites.Thenewplasmid.pUC19一 lib.Tvector.wasdigestedwithmItoderiveaT.vector with3endoverhangingaTbase.1ResultlTheconstructedpUC19一HB—...