The circular permutation assay has been used to characterize sequence-dependent bending in plasmid pUC19 (2686 bp). Linear, permuted sequence isomers of pUC19 exhibit different mobilities in large-pore polyacrylamide gels, suggesting that the plasmid contains two sequence-dependent bends, one located at...
[Method]pUC192CMwasconstructedbyinsertingachloramphenicolresistantgeneintothemulti2cloningsiteofpUC19, thentwohomologousfragmentswereclonedintoeachsideofthepUC192CMtoconstructsuicideplasmidpUC192CM2D.Areplacementmutant strain,whosetargetgenewasreplacedbyresistantgene,couldbeobtainedbytransformingthesuicideplasmidpUC192CM2D...
Kittler L,L?ber G.Sequence specificity of DNA-psoralen photoproduct formation in supercoiled plasmid DNA (pUC19).Journal of Photochemistry and Photobiology. B: Biology. 1995Kittler L,L?ber G.Sequence specificity of DNA-psoralen photoproduct formation in supercoiled plasmid DNA (pUC19). Journal of ...
‘plasmid’. they have several restriction sites. let’s look at the difference between these two vectors. pbr322 it was the first artificial cloning vector created in the laboratory by francisco bolivar and raymond l.rodriguez. also, pbr322 is bigger in size compared to its puc counterparts....
英文名称:GAS GFP Reporter Plasmid 规格:1μg GAS-GFP报告基因是用于检测STAT1/STAT1转录活性水平为目的的报告基因。GAS(gamma-activated sequence, GAS) 又称γ干扰素活化序列在皮肤发生迟发型接触性过敏时,可与转位至角朊细胞核内的STAT-91结合而激活K17的表达。
[ Method] pUC19一 cM was constr ucted by inserting a chloramphenicol resistant gene into the multi— cloning site of pUCI9 , then tw o homol ogous fragments were cl oned into eac h side of the pU C 19 一 C M to constr u c t sui ci de plasmid pU C 19一 C M — D .A rep...
Kittler L,L?ber G.Sequence specificity of DNA-psoralen photoproduct formation in supercoiled plasmid DNA (pUC19). Journal of Photochemistry and Photobiology. B: Biology . 1995Kittler L,L?ber G.Sequence specificity of DNA-psoralen photoproduct formation in supercoiled plasmid DNA (pUC19). Journal ...