Genomic DNA from strain 70 was cloned in plasmid vector pUC19, and 18 random clones containing low-copy DNA sequences were used to probe seven strains ... RK Kulkarni - 《Appl.environ.microbiol》 被引量: 75发表: 1991年 Genetic tagging, cloning, and DNA sequence of the Synechococcus sp. ...
(1995). Sequence specificity ofDNA- psoralen photoproduct formation in supercoiled plasmid DNA (pUC19). Photochem. Photobiol. 27,161 -166.Kittler L,L?ber G.Sequence specificity of DNA-psoralen photoproduct formation in supercoiled plasmid DNA (pUC19). Journal of Photochemistry and Photobiology. B:...
[ Method] pUC19一 cM was constr ucted by inserting a chloramphenicol resistant gene into the multi— cloning site of pUCI9 , then tw o homol ogous fragments were cl oned into eac h side of the pU C 19 一 C M to constr u c t sui ci de plasmid pU C 19一 C M — D .A rep...
参考文献部分: Production of single-stranded plasmid DNA. Vieira J, Messing J. Methods Enzymol. 1987;153:3-11. PubMed 3323803 载体图谱质粒图谱和多克隆位点信息 载体简介 载体序列 LOCUS pUC119 3162 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION pUC cloning vector. ACCESSION . VERSION . KEYWORDS...
fragilis with the chimeric plasmids was dependent upon the source of the DNA; generally 10(5) transformants micrograms-1 of DNA were recovered when plasmid purified from B. fragilis was used. When the source of ... CJ Smith - 《Journal of Bacteriology》 被引量: 116发表: 1985年 Cloning ...
• For pUC18 DNA sequence, pUC19 DNA sequence, sequence analysis and map creation, see free online REviewer tool.Applications• Cloning• Sequencing of insert DNA, pUC18 DNA: Preparation of DNA molecular weight standardspUC18/19 plasmid contents and usage notes - pUC18/19 plasmids contain:•...
[Method]pUC192CMwasconstructedbyinsertingachloramphenicolresistantgeneintothemulti2cloningsiteofpUC19, thentwohomologousfragmentswereclonedintoeachsideofthepUC192CMtoconstructsuicideplasmidpUC192CM2D.Areplacementmutant strain,whosetargetgenewasreplacedbyresistantgene,couldbeobtainedbytransformingthesuicideplasmidpUC192CM2D...
respectively. Four plasmids except pFX conferred CPZ resistance. In order to search an ideal plasmid as the vector in cloning of cefoperazone resistance g ene (CPZr) from resistance plasmid pFC, we studied plasmid pUC19 which is used widely in molecular cloning. pUC19 was transformed into E.col...
This DNA is a plasmid vector suitable for DNA sequencing by dideoxy method. This DNA may allow successful cloning of DNA fragment longer than the case of M13 phage vector used. Since it has multi-cloning sites onlacZ'region, any DNA insertion into this vector can be easily verified using pl...
pUC19 is a small, high-copy numberE. coliplasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), which allows for blue/white colony ...