coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC18 vector except that it is arranged in opposite orientation.Highlights• Purified by chromatography using proprietary patented technology• More than 90% in the supercoiled form• Isolated from E.coli (...
Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. Usage Suggestion:The ORF cDNA sequence can be amplified by PCR with M13-47 and RV-M primers. pUC19 Vector Physical Map Download sequence Notes: 1. The full-length cDNA sequence included of 5' UTR and ...
The circular permutation assay has been used to characterize sequence-dependent bending in plasmid pUC19 (2686 bp). Linear, permuted sequence isomers of pUC19 exhibit different mobilities in large-pore polyacrylamide gels, suggesting that the plasmid contains two sequence-dependent bends, one located at...
• For pUC18 DNA sequence, pUC19 DNA sequence, sequence analysis and map creation, seefree online REviewer tool.Applications• Cloning• Sequencing of insert DNA, pUC18 DNA: Preparation of DNA molecular weight standardspUC18/19 plasmid contents and usage notes - pUC18/19 plasmids contain:• ...
Monarch® Plasmid Miniprep Kit Monarch® DNA Gel Extraction Kit Monarch® PCR & DNA Cleanup Kit (5 μg) 参考文献 Yanisch-Perron, C., Vieira, J. and Messing, J. (1985). Gene. 33, 103-119. 工具& 资源 Web 工具 DNA Sequences and Maps Tool ...
• For pUC18 DNA sequence, pUC19 DNA sequence, sequence analysis and map creation, seefree online REviewer tool. Applications • Cloning • Sequencing of insert DNA, pUC18 DNA: Preparation of DNA molecular weight standards pUC18/19 plasmid contents and usage notes - pUC18/19 plasmids contain...
英文名称:GAS GFP Reporter Plasmid 规格:1μg GAS-GFP报告基因是用于检测STAT1/STAT1转录活性水平为目的的报告基因。GAS(gamma-activated sequence, GAS) 又称γ干扰素活化序列在皮肤发生迟发型接触性过敏时,可与转位至角朊细胞核内的STAT-91结合而激活K17的表达。
1995 . Sequence specificity of DNA-psoralen photoproduct formation in supercoiled plasmid DNA (pUC19). J. Photochem. Photobiol. B 27: 161 – 166 .Kittler L,L?ber G.Sequence specificity of DNA-psoralen photoproduct formation in supercoiled plasmid DNA (pUC19). Journal of Photochemistry and ...
‘plasmid’. they have several restriction sites. let’s look at the difference between these two vectors. pbr322 it was the first artificial cloning vector created in the laboratory by francisco bolivar and raymond l.rodriguez. also, pbr322 is bigger in size compared to its puc counterparts....
pUC19 Vector Information Description pUC19 is a small, high-copy numberE. coliplasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686 base pairs in length. pUC19 encodes the N-terminal fragment of b-galactosidase (lacZa), wh...