Through RNA immunoprecipitation, dual‐luciferase reporter assay and RNA stability detection, we confirmed that PTBP1 binds to SLC31A1 mRNA and regulates the expression level of SLC31A1 by affecting mRNA stability. Additionally, SLC31A1 silencing abrogated the chemosensitizing effect of PTBP1 knock‐...
Moreover, we also demonstrate that PTBP1, but not PTBP2, inhibits the expression of AXL mRNA and the RNA recognition motif 1 (RRM1) of PTBP1 is crucial for this interaction. To clarify how PTBP1 regulates AXL expression at the mRNA level, we found that, while the transcription rate of...
PTBP1作为一种RNA结合蛋白,参与调控mRNA的剪接、翻译、稳定性和定位等多种细胞内生物过程,与肿瘤关系极为密切。在肿瘤细胞中PTBP1与多种非编码RNA相互作用影响肿瘤的进展和治疗,这提示PTBP1可能是协调非编码RNA发挥作用的关键因子之一。但当前PTBP1与各类非编码RNA的...
PTBP1 is a common RNA-binding protein that plays an important role in the occurrence and development of a variety of diseases. Studies have shown that PTBP1 in breast cancer and lung cancer cells can increase the metastatic power and invasion ability of breast cancer and lung cancer cells by...
PTBP1, as a key splicing factor, regulates the splicing of target gene precursor messenger RNA(mRNA) and the stability of mRNA, and its novel molecular mechanism for regulating alternative splicing has also been further elucidated in digestive tract tumors. In this paper, the research progress on...
多聚嘧啶区结合蛋白1(PTBP1)重组蛋白 Recombinant Polypyrimidine Tract Binding Protein 1 (PTBP1)HNRNPI; HNRPI; PTB; PTB-T; PTB2; PTB3; PTB4; pPTB; Heterogeneous Nuclear Ribonucleoprotein I; 57 kDa RNA-binding protein PPTB-1 [ PROPERTIES ]Source: Prokaryotic expressionHost: E.coli...
Dysfunction of podocytes has been regarded as an important early pathologic characteristic of diabetic kidney disease (DKD), but the regulatory role of long noncoding RNAs (lncRNAs) in this process remains largely unknown. Here, we performed RNA sequenci
Recent studies also confirmed that circRNAs can interact with RNA-binding proteins (RBPs) to modulate their interacted mRNA stability and function [4,5,6,7,8]. However, the mechanisms of circRNAs in CRC remain poorly understood. Cancer-derived exosomes are small vesicles (30–100 nm in ...
Nuclear RBPs mainly process precursor mRNAs, including RNA modifications and alternative splicing. Moreover, cytoplasmic RBPs mainly regulate mRNA transport, controlling mRNA stability and interactions with the translational machinery [9]. Recently, more studies have indicated that RBPs participate in the...
Binding of PTBP1 not only facilitates the stability of MACC1-AS1 and enhances the sponge effect of MACC1-AS1 on miRNAs, but also competes with PTBP1 target mRNAs for PTBP1 binding. Our results reveal that the two trans-acting regulators, PTBP1 and miRNA, coordinate for binding to lncRNA ...