所有设计规则的整合导致mRNAs同时具有高溶液稳定性和高蛋白质产量。 讨论 PDF原文 今天给各位翻译的是2022年《NATURE COMMUNICATIONS》期刊文章:Combinatorial optimization of mRNA structure, stability, and translation for RNA-based therapeutics。 摘要 针对包括新冠肺炎在内的多种人类疾病的治疗性mRNAs和疫苗正在开发中...
3. LindeSchoenmaker, Dominik Witzigmann, Jayesh A. Kulkarni, Rein Verbeke, GideonKersten, Wim Jiskoot, Daan J.A. Crommelin. mRNA-lipid nanoparticle COVID-19vaccines: Structure and stability.Int. J. Pharm.2021, 601, 120586.
3. LindeSchoenmaker, Dominik Witzigmann, Jayesh A. Kulkarni, Rein Verbeke, GideonKersten, Wim Jiskoot, Daan J.A. Crommelin. mRNA-lipid nanoparticle COVID-19vaccines: Structure and stability. Int. J. Pharm. 2021, 601, 120586.
3. LindeSchoenmaker, Dominik Witzigmann, Jayesh A. Kulkarni, Rein Verbeke, GideonKersten, Wim Jiskoot, Daan J.A. Crommelin. mRNA-lipid nanoparticle COVID-19vaccines: Structure and stability.Int. J. Pharm.2021, 601, 1205...
and stability, both in solution and in cells. For example, it has typically been assumed that mRNAs with more stable secondary structure might have increased in-solution stability but would have lower in-cell protein output due to the increased difficulty of the cellular translation machinery to ...
These models are not mutually exclusive, but merely provide a physicochemical view on what features of the structure and stability of the pseudoknot, relative to a simple hairpin, may induce the ribosome to pause, and thus increase the likelihood of shifting reading frames. Other more complex ...
题目:Sequence- and structure-selective mRNA mCmethylation by NSUN6 in animals Natl Sci Rev(IF 16.693),PubDate: 2021 Jun 单位:中山大学孙逸仙纪念医院生命科学学院RNA生物医学研究所基因功能与调控教育部重点实验室,生物防治国家重点实验室 分子机制:mRNAm5C在各种生物事件中发挥着重要的调节作用。在 mRNA 中发...
A-to-I editing is catalysed by adenosine deaminases acting on RNA (ADARs) and can change RNA structure and stability. A-to-I editing can also introduce mutations into proteins as ‘I’ bases are read as guanosines instead of adenosines during translation. Autophagy A lysosome-dependent ...
解析出家蚕R2型non-LTR逆转录转座子在核糖体靶标DNA处起始靶向引导的反转录 (TPRT) 的冷冻电镜结构,结构显示其与靶标DNA存在一个广泛的接触界面,其3’非翻译区 (UTR) 上一段小的核心区域是TPRT必需的,改造R2RM可改变其插入位点,上述结果已发表在Science杂志,题为:Structure of the R2 non-LTR ...
2.4mRNA stability mRNA stability is very crucial for optimum expression of a protein in any host cell. Several factors such as mRNAsecondary structure, 5′ and 3′untranslated region(UTR) sequences, mRNA length, 5′ Cap, 3′ polyA tail,microRNA(miRNA),codon usageand many other factors determ...