薛愿超课题组开发了CRIC-seq新技术,并利用该技术直接证明了PTBP1可通过介导跨越可变外显子的RNA环而抑制剪接,率先揭示了PTBP1通过介导内含子内部RNA成环从而促进可变外显子剪接的新机制。 已知,RBP可以结合、稳定或破坏RNA-RNA相互作用。一些模型提出RBP可通过介导RNA-RNA远距离互作而参与可变剪接调控,但缺乏直接的实验...
Collectively, these results indicate that exon 9 possesses splicing regulatory activity that is partially separable from the repressive activity conferred by RRM2 and that skipping of exon 9 reduces the negative and positive regulatory activities of PTBP1 without substantially affecting RNA binding activity...
多聚嘧啶区结合蛋白1(polypyrimidine tract-binding protein 1,PTBP1)是一种常见的RNA结合蛋白,广泛表达在神经细胞和非神经细胞,参与调节多种生物学过程[3]。之前的研究发现使用CRISPR-CasRx系统敲低Ptbp1,能够诱导胶质细胞转化为神经元[4]。也有研究发现使用shRNA或反义寡核苷酸(ASO)敲低Ptbp1蛋白表达,能够将星形...
多聚嘧啶区结合蛋白1(polypyrimidine tract-binding protein 1,PTBP1)是一种常见的RNA结合蛋白,广泛表达在神经细胞和非神经细胞,参与调节多种生物学过程[3]。之前的研究发现使用CRISPR-CasRx系统敲低Ptbp1,能够诱导胶质细胞转化为神经元[4...
Regulation of alternative splicing events is an essential step required for the expression of functional cytoskeleton and sarcomere proteins in cardiomyocytes. About 3% of idiopathic dilated cardiomyopathy cases present mutations in the RNA binding protein RBM20, a tissue specific regulator of alternative ...
据报道,当polypyrimidine tract-binding protein 1(PTBP1,一种参与RNA生物学的因子)被抑制时可迅速诱导GtN转换,然后成为精确连接的成熟神经元,并减轻神经系统疾病和衰老相关认知障碍的症状。这些惊人的发现,如果得到证实,将构成再生医学的重大进步。然而,GtN转化和通过PTBP1抑制的治疗潜力都没有被采用严格方法的多个重复...
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA pro...
PTBP1 preferentially binds to polypyrimidine-rich stretches of RNA. It functions mainly in splicing, and can shuttle between the cytoplasm and the nucleus [7]. PTBP1 is a common RNA-binding protein that plays an important role in the occurrence and development of a variety of diseases. Studies...
Polypyrimidine tract binding protein 1(PTBP1), a kind of RNA binding protein, is one of the members of the splicing factor heterogeneous nuclear ribonucleoproteins(hnRNPs) family, and is involved in the occurrence and progression of various tumors through regulation of proliferation, migration, ...
2022年6月14日，西奈山伊坎医学院的陈波团队在Cell Reports发表论文“Critical examination of Ptbp1-mediated glia-to-neuron conversion in the mouse retina”，表明通过CRISPR-CasRx或shRNA下调Ptbp1不足以将Mϋller glia（MG）转分化为视网膜神经节细胞（retinal ganglion cells,RGC）。2022年9月21日，厦门大学...