So far, little is known about how defective, mislocalized, or abnormally accumulated membrane proteins are actively removed from the INM, especially in plants and animals. Here, via analysis of a proximity-labeling proteomic profile of INM-associated proteins in Arabidopsis, we identify critical ...
(2024). TurboID-Based Proximity Labeling: A Method to Decipher Protein–Protein Interactions in Plants. In: Fontes, E.P., Mäkinen, K. (eds) Plant-Virus Interactions. Methods in Molecular Biology, vol 2724. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3485-1_19 Download...
China Agricultural University. Described here is a proximity labeling method for identification of interaction partners of the TIR domain of the NLR immune receptor in Nicotiana benthamiana leaf tissue. Also provided is a detailed protocol for the ident
The above examples demonstrate the utility of PUP-IT as an effective proximity labeling system in plant biology. We next employed the PUP-IT system to investigate the membrane-based CELLULOSE SYNTHASE (CESA) complex (CSC), which underpins cellulose synthesis in plant cell wall biology15. CSC has...
Given these advantages, this article provides a detailed introduction to the principles of proximity labeling techniques and the development of labeling enzymes. The focus is on summarizing the recent applications of TurboID and miniTurbo in mammals, plants, and microorganisms.#D-8DQMfpJtz9TZZfzFbSsi...
This protocol describes the use of TurboID and split-TurboID in proximity labeling applications for mapping protein–protein interactions and subcellular proteomes in live mammalian cells. TurboID is an engineered biotin ligase that uses ATP to convert biotin into biotin–AMP, a reactive intermediate th...
In vivo interactome profiling by enzyme‐catalyzed proximity labeling 2021, Cell and Bioscience Visualizing protein–protein interactions in plants by rapamycin-dependent delocalization 2021, Plant Cell Establishment of proximity-dependent biotinylation approaches in different plant model systems ...
Proximity labeling provides a powerful in vivo tool to characterize the proteome of subcellular structures and the interactome of specific proteins. The nematode Caenorhabditis elegans is one of the most intensely studied organisms in biology, offering many advantages for biochemistry. Using the highly ...
KF Cho,TC Branon,S Rajeev,T Svinkina,AY Ting 摘要: Proximity labeling catalyzed by promiscuous enzymes, such as TurboID, have enabled the proteomic analysis of subcellular regions difficult or impossible to access by conventional fractionation-based approaches. Yet some cellular regions, such as ...
Our study illustrates that PL-MS using TurboID is a powerful tool for mapping signaling networks, and reveals broad roles of BIN2 kinase in cellular signaling and regulation in plants. Impact Statement TurboID-mediated proximity labeling is a powerful tool for protein interactomics in plants. 展开 ...