Proteinase 3-antineutrophil cytoplasmic antibody (PR3-ANCA) titer was elevated at 183 ELISA units (EU) in sera (normal range less than 10 EU), myeloperoxidase-ANCA was negative. PR3-ANCA titer was 250 and 1,070 EU in pleural effusions on right and left side, respectively. Although cessation...
As with all therapeutic proteins, there is potential for immunogenicity. No anti-A1PI antibodies have been detected in clinical trials of ZEMAIRA. The detection of antibody formation is highly dependent on the sensitivity and specificity of the assay. Additionally, the observed incidence of antibody ...
matriptase or trypsin (all 10 nM) for 16 hours at 37 °C. Incubation products were separated on a 7.5% SDS-PAGE gel and western blotted using an antibody recognising the aggrecan G1 domain. Results are
Once initiated, therapy will usually be continued for the remainder of the patient's life. Recipients of alpha 1-antitrypsin inhibitor therapy should be immunized against hepatitis B. It is also recommended that this medication not be used in patients with immunoglobulin antibody IgA deficiency that ...
Detection of the poorly protease-resistant PrPSc in VPSPr can require particular antibodies such as 1E4 (rather than by the 3F4 anti-PrP antibody) or enrichment after PK treatment [10, 11] for its detection by Western blotting. Nevertheless, in VPSPr, spongiform change and the widespread ...
Proteinase-activated receptors (PARs) are a subfamily of G protein-coupled receptors (GPCRs) with four members, PAR1, PAR2, PAR3 and PAR4, playing critical functions in hemostasis, thrombosis, embryonic development, wound healing, inflammation and cancer
This effect was neutralized when the 60-min SGF digestate was added along with anti-human IL-8/CXCL8 antibody (1.51 Ϯ 0.03; p ϭ 0.96). The cell proliferation ELISA was also run with PBS (1.58 Ϯ 0.10; p ϭ 0.80) and SGF (1.59 Ϯ 0.10; p ϭ 0.71) controls, which were...
Figs.3 and 4show that type I procollagen is readily cleaved by BMP-1myc. We wanted to know whether BMP-1myc cleaved procollagen at the physiological site. We used a neoepitope antibody that recognized the N-terminal 10 residues of the α1(I) chain C-propeptide. Western blot analysis ...
extending from approximately 8 kDa to the 18–30 kDa molecular mass range. Detection of the poorly protease-resistant PrPScin VPSPr can require particular antibodies such as 1E4 (rather than by the 3F4 anti-PrP antibody) or enrichment after PK treatment [10,11] for its detection by Western...