The anti-PR3 antibody鈥搃nduced oxidative burst of neutrophils was inversely correlated with the sialylation levels of anti-PR3 IgG.ConclusionThe sialylation level of anti-PR3 antibodies contributes to the clinical activity of GPA, by modulating the oxidative burst of neutrophils induced by these ...
s10a). We also investigated whether neutrophil elastase (NE) and cathepsin G (CatG), which are active forms of neutrophil serine proteinases like PRNT3, could interact with STAT3. Specifically, we added STAT3 antibody into HL60 cell lysates for immunoprecipitation with anti-NE and anti-CatG ...
The production level is optimal when the recombinant plasmid carries the TDH promoter, the complete 2μ and the leu2D selection marker. Biologically active recombinant α 1-PI can be purified either analytically, by affinity chromatography using a monoclonal antibody, or on a large scale, by a ...
Commercial assays for the diagnosis of hepatitis A detect antibody to hepatitis A virus (anti-HAV), but they cannot discriminate between antibody resulting... Yamina Kabrane-Lazizi a,Suzanne U Emerson a,Christian Herzog b,... - 《Vaccine》 被引量: 26发表: 2001年 A SIMPLE DOT IMMUNOASSAY FO...
Expression of p21 or p21A45R protein was investigated by means of Western blot analysis using a rabbit polyclonal anti-p21 antibody. Under basal conditions, p21 expression was higher in both U937/p21 and U937/p21A45R, as compared with control U937/PRCRSV. This was in agreement with the ...
Once initiated, therapy will usually be continued for the remainder of the patient's life. Recipients of alpha 1-antitrypsin inhibitor therapy should be immunized against hepatitis B. It is also recommended that this medication not be used in patients with immunoglobulin antibody IgA deficiency that...
To analyse the expression of the AccSp10 gene at the protein level, total protein was extracted from A. cerana cerana at four different pupal developmental stages following treatment with microorganisms and at 24 °C. Anti-AccSp10 serum was used as the primary antibody at a 1:400 (v/v) di...
As with all therapeutic proteins, there is potential for immunogenicity. No anti-A1PI antibodies have been detected in clinical trials of ZEMAIRA. The detection of antibody formation is highly dependent on the sensitivity and specificity of the assay. Additionally, the observed incidence of antibody ...
Down-regulation of GM-CSF expression by 3CLpro in A549 cells may be attributed in part to the reduced protein level of NF-kB (Figure 6). In addition, there were no significant decreases in the mRNA expression of IL-1β, IL-6, IL-8, IL12p40, TNF-α, TGF-β using RT-MPCR. This...
If a chimeric C-proteinase expressing a heterologous epitope has been used, detection of the peptide/C-proteinase complex may be accomplished by using a labeled epitope specific antibody. Once isolated, the identity of the peptide attached to the solid phase support may be determined by peptide ...