the cells were incubated with the primary antibody, mouse monoclonal IgG anti-GATAD1 (sc-81092, 1:100, Santa Cruz), in a blocking solution for 1 h. After washing the cells three times for 5 min with
the cells were incubated with the primary antibody, mouse monoclonal IgG anti-GATAD1 (sc-81092, 1:100, Santa Cruz), in a blocking solution for 1 h. After washing the cells three times for 5 min with
Protein nanoparticles usually perform MRI imaging by carrying magnetic nanoparticles or MRI contrast agents. Tao et al. [175] modified iron oxide nanoparticles with BSA and macromolecule (poly(acrylic acid)-poly(methacrylic acid), PMAA-PTTM) respectively to compare their MRI contrast performances. Afte...
The PA-TM-RING family (11 members in humans) is characterized by three conserved domains, including a protease-associated (PA) domain, a TM domain, and a RING-H2 domain.27The PA domain is responsible for protein-interacting. These endosomal membrane proteins appear short-lived and expressed at...
Acetylated peptides were enriched via immunoaffinity purification (IAP) using commercial antibody beads (Cell Signaling Technology, PTMScan Acetyl-Lysine Motif Kit) following the manufacturer’s instructions with minor adaptions. Initially, antibody beads were washed three times with IAP buffer (50 mM MO...
proteins are phosphorylated and investigate this using approaches such as cloning, 1D or 2D gel electrophoresis combined with Pro-Q Diamond staining [182–190], radioactive labelling using 32P or 33P [182, 183], or directed antibody-based strategies limited by their low specificity and high cost...
cells were incubated with anti-β-catenin antibody (#2677, 1:100, Cell Signaling) at 4 °C overnight. The next day, cells were incubated with Alexa Fluor® 488-conjugated anti-rabbit secondary antibody (#4412, 1:500, Cell Signaling) for 1 h at room temperature in the dark. DAPI...
mediated by the cooperation of cohesin and CTCF45. The involvement of ZNF143 in this complex has also been reported44. ZNF143 is believed to provide sequence specificity for chromatin interactions52. Overall, our analysis successfully recovered this important and well-characterized loop-forming complex...
expression of B cell lymphoma 6 (BCL6) by decreasing DNA methylation and H3K27me3 levels, thus enhancing Tfh cell differentiation, which cell group may be involved in the dysregulated antibody responses associated with SLE. The field requires further data to determine how RNF106 modulates the ...
6. The antibody of claim 1, 2, 3 or 4 wherein said antibody is conjugated to a fluorescent dye.Description: This is a National Stage Application of International Application No. PCT/US04/42940, filed Dec. 21, 2004 which is incorporated herein by reference in its entirety. FIELD OF THE ...