To further characterize the interactome of WT and mutated GATAD1 we used affinity purification-mass spectrometry (AP-MS) using the FLAG tag54. To this end, we generated stable inducible Flp-InTM-293 cells expressing GATAD1 fused to the promiscuous biotin ligase BirA* and FLAG tag in the N...
To further characterize the interactome of WT and mutated GATAD1 we used affinity purification-mass spectrometry (AP-MS) using the FLAG tag54. To this end, we generated stable inducible Flp-InTM-293 cells expressing GATAD1 fused to the promiscuous biotin ligase BirA* and FLAG tag in the N...
Because AID possesses an intrinsic affinity for RNA, protein complexes related to RNA processing might easily interact with AID in co-IP experiments or during affinity purification. In addition, SPT5- and SPT6-like proteins are able to form a macromolecular complex, because they associate withexoso...
Cai et al. [177] conjugated an MRI T1 contrast agent Gd-DTPA to the surface of H-ferritin nanoparticles. The T1 weight signal at the tumor site was significantly enhanced only 10 min after intravenous injection. Hu et al. [178] used the cavity of Physalis mottle virus (PhMV)-like nanop...
Acetylated peptides were enriched via immunoaffinity purification (IAP) using commercial antibody beads (Cell Signaling Technology, PTMScan Acetyl-Lysine Motif Kit) following the manufacturer’s instructions with minor adaptions. Initially, antibody beads were washed three times with IAP buffer (50 mM MO...
On the other hand, the second Zn finger appears to have no influence on iron binding, as the noZnF2 mutants had spectroscopic features identical to those of the parental WT or 4S proteins. These results strongly suggest that [2Fe-2S] binding requires the presence of at least one of two ...
As there is no antibody specific to p110 CUX1 only, an alternatestrategy must be employed to identify its targets. Results We expressed physiological levels of a tagged-p110 CUX1 protein and performedchromatin affinity purification followed by hybridization on ENCODE andpromoter arrays. Targets were...
As there is no antibody specific to p110 CUX1 only, an alternatestrategy must be employed to identify its targets. Results We expressed physiological levels of a tagged-p110 CUX1 protein and performedchromatin affinity purification followed by hybridization on ENCODE andpromoter arrays. Targets were...
Affinity purification experiments using P83916 (CBX1), Q99496 (RNF2), P16104 (H2AFX), and Q09028 (RBBP4) as baits. (A)Immunoblot confirming the expression of the indicated FLAG-tagged chromatin proteins using antibody against the 3X FLAG epitope. Two independent FLAG tag constructs of each...
Walker. et al., “Interaction of Human IgG Chimeric Antibodies With the Human FcRII Receptors: Requirements for Antibody-Mediated Host Cell-Target Cell Interaction” Molecular Immunology , vol. 26 No. 4, pp. 403-411 1989. Dalva, et al “EphB Receptors Interact with NMDA Receptors and Regula...