the cells were incubated with the primary antibody, mouse monoclonal IgG anti-GATAD1 (sc-81092, 1:100, Santa Cruz), in a blocking solution for 1 h. After washing the cells three times for 5 min with
and Q09028 (RBBP4) as baits. (A)Immunoblot confirming the expression of the indicated FLAG-tagged chromatin proteins using antibody against the 3X FLAG epitope. Two independent FLAG tag constructs of each chromatin related protein was constructed for affinity purifications to eliminate background...
mediated by the cooperation of cohesin and CTCF45. The involvement of ZNF143 in this complex has also been reported44. ZNF143 is believed to provide sequence specificity for chromatin interactions52. Overall, our analysis successfully recovered this important and well-characterized loop-forming complex...
产品别名: ECK4026 antibody JW3994; Mal E; MalE; malJ; Maltodextrin binding protein; Maltose ABC transporter periplasmic protein; Maltose binding periplasmic protein; MBP; MMBP; Periplasmic maltose binding protein. 英文名称:Anti-Maltose Binding Protein/MBP ...
A FLAG-tagged overexpression plasmid was transfected into 293 T cells, and the same lysate was used to blot FLAG as that used in the immunoblotting of AXIN1. The AXIN1 antibody (CST) could not only recognize the full length of AXIN1 but also detect the overexpressed AXIN1-295aa that ...
Affinity purification experiments using P83916 (CBX1), Q99496 (RNF2), P16104 (H2AFX), and Q09028 (RBBP4) as baits. (A)Immunoblot confirming the expression of the indicated FLAG-tagged chromatin proteins using antibody against the 3X FLAG epitope. Two independent FLAG tag constructs of each...
ZNF32 antibody was produced and purified as previously described27, and used in a dilution of 1:50. Horseradish peroxidase-conjugated secondary antibody to rabbit IgG (1:5000, Santa), and horseradish peroxidase-conjugated secondary antibody to mouse IgG (1:8000, Santa). The membrane was ...
expression of B cell lymphoma 6 (BCL6) by decreasing DNA methylation and H3K27me3 levels, thus enhancing Tfh cell differentiation, which cell group may be involved in the dysregulated antibody responses associated with SLE. The field requires further data to determine how RNF106 modulates the ...
ZNF143 is believed to provide sequence specificity for chromatin interactions52. Overall, our analysis successfully recovered this important and well-characterized loop-forming complex. The other two cliques, PML-FOXM1-MTA3-STAT5A-CEBPB- RUNX3 and MAX-MAZ-MXI1-CHD2-BHLHE40 (Fig. 3c) have not...
Sections were hybridized for 20 h at 42 °C with hybridization probe specific to CXXC4 and were incubated with biotinylated anti-digoxin antibody. To visualize the signal, the slides were incubated with 3,3′-diaminobenzidine tetrahydrochloride hydrate (Genentech, San Francisco, CA, USA) for ...