the cells were incubated with the primary antibody, mouse monoclonal IgG anti-GATAD1 (sc-81092, 1:100, Santa Cruz), in a blocking solution for 1 h. After washing the cells three times for 5 min with
the cells were incubated with the primary antibody, mouse monoclonal IgG anti-GATAD1 (sc-81092, 1:100, Santa Cruz), in a blocking solution for 1 h. After washing the cells three times for 5 min with
UDP-galactose + glucose ⇌UDP+ lactose. Thus it can be seen that the presence of the α-lactalbumin sub-unit changes the specificity of the enzyme, causing it to transfergalactoseto glucose rather than toN-acetylgiucosamine. The overall process involves conformational changes in the region of ...
and Q09028 (RBBP4) as baits. (A)Immunoblot confirming the expression of the indicated FLAG-tagged chromatin proteins using antibody against the 3X FLAG epitope. Two independent FLAG tag constructs of each chromatin related protein was constructed for affinity purifications to eliminate background...
Although alterations affecting genes in the UPS pathway would be expected to lead to multiple changes in downstream protein substrates, mutations in the substrates themselves could provide greater specificity for cancer cells by affecting much fewer proteins. We therefore hypothesized that we could identif...
mediated by the cooperation of cohesin and CTCF45. The involvement of ZNF143 in this complex has also been reported44. ZNF143 is believed to provide sequence specificity for chromatin interactions52. Overall, our analysis successfully recovered this important and well-characterized loop-forming complex...
Cell-based assays andmolecular studies have revealed that transcriptional regulation byCUX1 involves mostly the proteolytically processed p110isoform. As there is no antibody specific to p110 CUX1 only, an alternatestrategy must be employed to identify its targets. Results We expressed physiological ...
Cell-based assays andmolecular studies have revealed that transcriptional regulation byCUX1 involves mostly the proteolytically processed p110isoform. As there is no antibody specific to p110 CUX1 only, an alternatestrategy must be employed to identify its targets. Results We expressed physiological ...
proteins are phosphorylated and investigate this using approaches such as cloning, 1D or 2D gel electrophoresis combined with Pro-Q Diamond staining [182–190], radioactive labelling using 32P or 33P [182, 183], or directed antibody-based strategies limited by their low specificity and high cost...
Walker. et al., “Interaction of Human IgG Chimeric Antibodies With the Human FcRII Receptors: Requirements for Antibody-Mediated Host Cell-Target Cell Interaction” Molecular Immunology , vol. 26 No. 4, pp. 403-411 1989. Dalva, et al “EphB Receptors Interact with NMDA Receptors and Regula...