1 Antibody structure and function Since the origin of uni- and multi-cellular organisms, the release and secretion of anti-microbial peptides has played a key role in cell and organismal survival by driving cell:cell communication and pathogen evasion. Among the pathogen-targeting secreted proteins...
(2005) have created a yeast proteome library of C-terminally TAP-tagged proteins which allows for the more efficient purification of transmembrane and secreted proteins. Gelperin and colleagues’ collection makes use of the Gateway recombinational technology, which allows for the easy transfer of ...
Agent that specifically binds to human MTAP protein in an embedded biological sample, especially a polyclonal antibody or a monoclonal antibody produced by hybridoma cell line ATCC Accession No. PTA-5001doi:US7157551 B2Compositions and methods involving agents that specifically bind to methyl...
Most cited: TAPA1 (CD81) (NM_004356) Human Recombinant Protein, Nucleolin (NCL) (NM_005381) Human Recombinant Protein, NF-kB p65 (RELA) (NM_021975) Human Recombinant Protein, FGF2 (NM_002006) Human Recombinant Protein, LOX (NM_002317) Human Recombinant Protein. Abnova 9th Fl., No.108...
(TAP-MS) was used with PgAFP-Flag-Strep and AfAFP-Flag-Strep as bait protein to isolate potential interacting proteins of AFPs inA. flavus. A total of 76 and 58 candidate protein targets were identified, respectively. A subsequent comparative analysis of the proteomes identified 38 proteins ...
Both Sigma-Aldrich and Agilent also offer vectors encoding “tandem-affinity tags,” dual peptide sequences that can be used in a two-step purification strategy to yield highly purified protein. Sigma-Aldrich’s TAP system, for instance, couples both the FLAG and HA (hemagglutinin) tags to a...
19). To explore the effect of TN1 on the TaPYL function, we wondered whether TN1 affects the physical association between TaPYL-1D and TaPP2C-7A. To test this idea, we co-expressed TN1 with cLUC-TaPP2C-7A and nLUC-TaPYL-1D proteins in N. benthamiana leaves. As expected, the LUC ...
The sections were then incubated with hematoxylin staining solution for 2 min, and sealed after rinsing with tap water for 15 min. The stained sections were observed and digitally photographed using a Leica DM2000 LED microscope. Immunofluorescence staining The intestinal sections were first de...
Luciferase activity. To measure the transcriptional activity of STAT1, we cotransfected cells with a TAP1 promoter luciferase-reporter construct in which the B site had been inactivated (a generous gift of D. Johnson, National Institutes of Health, Bethesda, Md.), together with the bidirectional ...
The function of Dbf2-Mob1 in cytokinesis is unclear. Also unknown is how Dbf2-Mob1 ultimately leads to release of Cdc14 from the nucleolus during mitotic exit. To give us insight into these two key cell cycle processes, we sought to identify potential substrates and phosphorylation sites of...