We propose that the interaction of E7 with TAP-1 prevents TAP-1 antibody detection and efficient peptide transport, resulting in poor presentation of viral antigen on HPV-infected cells and thus failure to mount an effective immune-mediated prevention of disease recurrence....
Microfluidic platforms also successfully employed for the detection of low abundant single cells membrane protein.53,54 The cells in droplets were utilized as the solid phase of the immunoreaction owing to non-essential cell lysis for cell membrane protein detection. The resultant protein antibody compl...
” dual peptide sequences that can be used in a two-step purification strategy to yield highly purified protein. Sigma-Aldrich’s TAP system, for instance, couples both the FLAG and HA (hemagglutinin) tags to a protein of interest; protein isolation over an anti-FLAG antibody column, followed...
Detection and reduction of protein-A contamination in immobilized protein-A purified monoclonal-antibody preparations. J. Immunol. Methods 117, 83–89 (1989). CAS Google Scholar DePalma, A. Affinity labels for protein purification. Genet. Eng. Biotechnol. N. 35, 24–26 (2015). Google Scholar...
Metastatic colorectal cancer (CRC) remains a substantial problem for mortality and requires screening and early detection efforts to increase survival. Epithelial-mesenchymal transition (EMT) and circulation of tumor cells in the blood play important roles in metastasis. To identify a novel target for ...
R&D Systems also produces quality antibodies, antibody arrays, stem cell and cell culture products, and cell selection and detection products, serving the life science and diagnostics industry. Most cited: Recombinant Human TGF-beta 1 Protein, Recombinant Human TGF-beta 1 Protein, CF, Recombinant ...
1. Protein A Standard(proteinA蛋白标准品) Recombinant Alkali-tolerant Protein A in a protein matrix with preservative, 50μg/mL, 1x0.1mL Bk031 2-8°C 2. Biotinylated Antibody(生物素标记的抗体) Biotinylated anti-protein A monoclonal antibody in a protein matrix with preservative, 1x13mL ...
Luciferase activity. To measure the transcriptional activity of STAT1, we cotransfected cells with a TAP1 promoter luciferase-reporter construct in which the B site had been inactivated (a generous gift of D. Johnson, National Institutes of Health, Bethesda, Md.), together with the bidirectional ...
5D), reminiscent of the reduced proportion of S-phase TAPC in the aged hippocampus and decreased DCX-positive progenitor cells in the DG region (Figs. 1E and S3C). And 14 downregulated genes shared between cluster 5-specific DEGs and aged neuron-specific DEGs (ARPP21, NRG1, etc.) were ...
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