These functions are tightly regulated through the co-evolution of 2 separate protein-domains, the Fragment:antibody binding (Fab) and the Fragment:crystallizable (Fc) domain of an antibody, each responsible for directing the bi-functional activity of these molecules. Antibodies arose approximately 500...
Compositions and methods involving agents that specifically bind to methylthioadenosine phosphorylase (MTAP) protein are disclosed. Illustrative compositions comprise binding agents that bind to human MTAP protein in biological samples, including embedded samples. The binding agents are useful, for example,...
(2005) have created a yeast proteome library of C-terminally TAP-tagged proteins which allows for the more efficient purification of transmembrane and secreted proteins. Gelperin and colleagues’ collection makes use of the Gateway recombinational technology, which allows for the easy transfer of ...
Cited: Anti-GLUT1 RBD (receptor binding domain, can function as an antibody). Cell Signaling Technology 3 Trask LaneDanvers, MA 01923 info@cellsignal.com https://www.cellsignal.com 8776162355 headquarters: USA Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately...
Both Sigma-Aldrich and Agilent also offer vectors encoding “tandem-affinity tags,” dual peptide sequences that can be used in a two-step purification strategy to yield highly purified protein. Sigma-Aldrich’s TAP system, for instance, couples both the FLAG and HA (hemagglutinin) tags to a...
DNA–protein crosslinks (DPCs) induced by aldehydes interfere with replication and transcription. Hereditary deficiencies in DPC repair and aldehyde clearance processes cause progeria, including Ruijs–Aalfs syndrome (RJALS) and AMeD syndrome (AMeDS) in
How RNA-binding proteins (RBPs) convey regulatory instructions to the core effectors of RNA processing is unclear. Here, we document the existence and functions of a multivalent RBP–effector interface. We show that the effector interface of a conserved
(TAP-MS)TAP-MS is based on the double tagging of the protein of interest on its chromosomal locus, followed by a two-step purification process and mass spectroscopic analysis. Protein arrayA high-throughput method, it is used to track the interactions and activities of proteins and to determin...
Luciferase activity. To measure the transcriptional activity of STAT1, we cotransfected cells with a TAP1 promoter luciferase-reporter construct in which the B site had been inactivated (a generous gift of D. Johnson, National Institutes of Health, Bethesda, Md.), together with the bidirectional ...
Tuberculosis (TB) is a contagious disease that predominantly affects the lungs, but can also spread to other organs via the bloodstream. TB affects about o