Determining the three-dimensional structures of macromolecules is a major goal of biological research, because of the close relationship between structure and function; however, thousands of protein domains still have unknown structures. Structure determination usually relies on physical techniques including ...
To reveal the bioprocess mechanism, it is important to determine the native structure and intact assemblies of biomolecules in the physiological environment. X-ray crystallography is one of the most well-established techniques for structural biology; and more than 130 thousand biomolecule structures have...
We describe an experimental method of three-dimensional (3D) structure determination that exploits the increasing ease of high-throughput mutational scans. Inspired by the success of using natural, evolutionary sequence covariation to compute protein and
Insulin — gross molecular structure: trial-and-error studies using transform and Patterson function techniques Proc. Natl. Acad. Sci. U. S. A., 48 (1962), p. 2150 CrossrefGoogle Scholar [90] J.R. Einstein, A.S. McGavin, B.W. Low Insulin. Some shrinkage stages of sulfate and citra...
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The gap between protein sequence and structure continues to enlarge rapidly as the throughput of genome sequencing techniques is much higher than that of protein structure determination techniques. Computational software tools for predicting protein structure and structural features from protein sequences are...
Protein structure determination from NMR chemical shifts Proc. Natl. Acad. Sci. U. S. A., 104 (23) (2007), pp. 9615-9620 Google Scholar [22] K. Chen, N. Tjandra The use of residual dipolar coupling in studying proteins by NMR NMR of Proteins and Small Biomolecules, Springer (2011)...
A series of breakthroughs in protein production and structure determination techniques, especially in protein crystallography, nuclear magnetic resonance and electron microscopy, have enabled researchers to solve the structure of macromolecular complexes and oligomeric proteins of very large size, sometimes ...
Mass SpectrometryPoster NoteNovel Ways to Introduce the Traditional Salt Based Chromatography Techniques of Size Exclusion and Ion Exchange Chromatography of Biopharmaceutical Proteins Into High Resolution Mass SpectrometryWorkflow Top DownPoster NoteOptimiz...
Despite the promising utility of HRPF, the covalent labeling data lacks detailed structural information sufficient for unambiguous tertiary structure determination18. The marriage of HRPF data and computational techniques provides a unique opportunity for more accurate protein structure prediction. The recent...