Evaluation of techniques to determine the protein digestibility of heat processed beans for pigs. Anim. Feed Sci. Technol. 33: 331-341.Vander Poel,A.F.B.,J.Doorenbos, J.Huisman and H.Boer., 1991.Evaluation oftechniques todetermine the protein digestibility of heat processed beans for pigs....
This is one of a kind as researchers determine protein structure from solid samples, that is, from crystals, using X-ray or from frozen solutions with the help of electron microscopy. Yet, at times, crystals are hard to obtain, and most importantly, proteins work in the liquid state where...
Determining the size of critical nuclei remains challenging. Here the authors propose a method to determine the size of critical secondary nuclei on growth faces of poly(butylene succinate) single crystals in solution, using the probability of statistically selecting crystallizable units in random copoly...
By functional assay: it is best to perform a functional assay to determine if any active protein is present. By immunoassay: by performing an immunoblot or ELISA, antibody test of structure determinant can reflect the real state of protein folding. ...
This thesis summarizes my effects to apply theoretical methods to understand the structure and function relationships of membrane proteins. Specifically, we developed computational techniques to interpret solution and solid-state NMR data of membrane proteins and determine their high resolution structures. ...
Structure determination of membrane proteins has historically been, and to a certain extent remains, a technically challenging endeavor – demonstrated by the fact that membrane proteins comprise less than 2% of all structures deposited in the Protein Data Bank (PDB) as of January 2020 [4]. These...
F. TUNEL assay and SCSA determine different aspects of sperm DNA damage. Andrologia 42, 305–313 (2010). Article PubMed Google Scholar Sergerie, M., Laforest, G., Bujan, L., Bissonnette, F. & Bleau, G. Sperm DNA fragmentation: threshold value in male fertility. Hum. Reprod. 20, ...
which has been adopted in many studies [122]. In addition, other cytokines such as HGF, IFN-α, and TNF-α have also been widely tested for their clinical value. In the last 5 years, most clinical trials have used enzyme-linked immunosorbent assay (ELISA) to determine protein levels, whi...
[213]. It is typically necessary to find a reasonable balance between these two aspects to meet varying clinical needs and objectives. In diagnosis, diagnostic tests with high specificity are typically used to determine whether a patient has a specific disease. However, highly sensitive circulating ...
3.2. Techniques to determine the active ingredients released from bioactive coatings UV–Vis spectroscopy is the most widely employed analytical technique to quantify the various components that are released from bioactive coatings [258]. Table 3 shows a summary of the drug classes, drugs, and wavele...