This is one of a kind as researchers determine protein structure from solid samples, that is, from crystals, using X-ray or from frozen solutions with the help of electron microscopy. Yet, at times, crystals are hard to obtain, and most importantly, proteins work in the liquid state where...
Evaluation of techniques to determine the protein digestibility of heat processed beans for pigs. Anim. Feed Sci. Technol. 33: 331-341.Vander Poel,A.F.B.,J.Doorenbos, J.Huisman and H.Boer., 1991.Evaluation oftechniques todetermine the protein digestibility of heat processed beans for pigs....
Nuclear magnetic resonance (NMR) is a powerful technique for protein structure determination in solution. However, when dealing with metalloproteins, NMR methods are unable to directly determine the structure of the metal site and its coordination geometry. The capability of X-ray absorption spectroscop...
MicroED can be used to determine the structure of protein-drug complexes and small molecules. Critically, MicroED alleviates the need for long and complicated large-crystal growth, a significant benefit in the fast-paced pharmaceutical industry. To highlight some of the key benefits of MicroED, we...
This thesis summarizes my effects to apply theoretical methods to understand the structure and function relationships of membrane proteins. Specifically, we developed computational techniques to interpret solution and solid-state NMR data of membrane proteins and determine their high resolution structures. ...
MicroED can be used to determine the structure of protein-drug complexes and small molecules. Critically, MicroED alleviates the need for long and complicated large-crystal growth, a significant benefit in the fast-paced pharmaceutical industry. To highlight some of the key benefits of MicroED, we...
By functional assay: it is best to perform a functional assay to determine if any active protein is present. By immunoassay: by performing an immunoblot or ELISA, antibody test of structure determinant can reflect the real state of protein folding. ...
sgRNA sequence to target DNA loci of interest. CRISPR/Cas is notably diverse in terms of structure and function, being generally divided into two classes according to the complexity of the protein effectors: multi-subunit effector complexes in class 1 or single protein effectors in class 2 [34]...
An antibody’s DNA and amino acid sequence heavily determine how it folds into a 3D shape. However, how the protein works can be dramatically affected by post-translational enzymatic or chemical changes, or the mis-formation of disulfide bonds.7 ...
(ACLT) dog cartilage, performed PCA to find the principle features and then adapted them to the FDA model to determine OA classification.44According to their findings, the classification succeeded with high accuracy for the training set (95.7%) and cross-validation (94.3%). Zhang et al. ...