doi:10.1016/0167-7799(87)90081-3Iain D. CampbellBrian SheardElsevier LtdTrends in BiotechnologyI. D. Campbell and B. Sheard, "Protein structure determination by NMR," Trends in Biotechnology, vol. 5, no. 11, pp. 302-306, 1987.
Using conventional triple-resonance nuclear magnetic resonance (NMR) experiments with a 1 mm triple-resonance microcoil NMR probe, we determined near complete resonance assignments and three-dimensional (3D) structure of the 68-residue Methanosarcina mazei TRAM protein using only 72 mu g (6 mu l,...
De novo determination of peptide structure with solid-state magic-angle spinning NMR spectroscopy The three-dimensional structure of the chemotactic peptide N-formyl-L-Met-L-Leu-L-Phe-OH was determined by using solid-state NMR (SSNMR). The set of SSNMR ... CM Rienstra,L Tucker-Kellogg,CP ...
Protein Structure Determination by Nuclear Magnetic Resonance Spectroscopy The power of nuclear magnetic resonance (NMR) spectroscopy for elucidation of structure and structure鈥揻unction relationships in proteins became evident shortly after the discovery of the NMR phenomenon. This chapter presents the ...
An approach for high-throughput structure determination of proteins by NMR spectroscopy An approach is described for rapidly determining protein structures by NMR that utilizes proteins containing 13 C-methyl labeled Val, Leu, and Ile (未1) an... A Medek,ET Olejniczak,RP Meadows,... - 《Journa...
NMR spectroscopy and cryo-electron microscopy. Here we present a method that allows the high-resolution three-dimensional backbone structure of a biological macromolecule to be determined only from measurements of the activity of mutant variants of the molecule. This genetic approach to structure determ...
Protein structure determination in solution by NMR spectroscopy. J. Biol. Chem. 265, 22059–22062 (1990). Article PubMed Google Scholar Slabinski, L. et al. The challenge of protein structure determination-lessons from structural genomics. Protein Sci. 16, 2472–2482 (2007). Article CAS Pub...
Structure determination of a new protein from backbone-centered NMR data and NMR-assisted structure prediction. Proteins-Structure Function And Bioinformatics. ... KL Mayer,Y Qu,S Bansal,... - Wiley Subscription Services, Inc., A Wiley Company 被引量: 0发表: 2006年 Structure and Interactions ...
Protein - Isolation, Determination, Structure: Animal material usually contains large amounts of protein and lipids and small amounts of carbohydrate; in plants, the bulk of the dry matter is usually carbohydrate. If it is necessary to determine the amou
In NMR studies, UL49.5 was represented by two fragments: the extracellular region (residues 1–35) and the transmembrane-intracellular fragment (residues 36–75), displaying various functions during viral invasion. After the empirical structure determination, a molecular docking procedure was used to ...