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protein interactionsolution NMR spectroscopyRecent advances in spectroscopic methods allow the identification of minute fluctuations in a protein structure. These dynamic properties have been identified as keys to some biological processes. The consequences of this structural flexibility can be far鈥憆eaching...
NMR spectroscopy is ideally suited for elucidating reaction mechanisms because it can simultaneously provide information on protein structure and reaction kinetics. Structural information is available primarily in the form of chemical shifts [20,21], but also through residual dipolar couplings [22], chemi...
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NMR spectroscopy is one of the most pivotal methods to study drug–target interactions in solution, because it is a robust technique to provide information on protein structure, dynamics, and interaction at an atomic level in solution, although it has the low absolute sensitivity. NMR spectroscopy...
The general approach to globular proteins dynamical structure investigation by NMR and time domain dielectric spectroscopy (TDDS) is presented. The information on macromolecular dynamical behavior in the case of these two methods is obtained in terms of correlation function and its parameters of atom mo...
Towards Automating Protein Structure Determination from NMR Data Nuclear magnetic resonance (NMR) spectroscopy technique is becoming exceedingly significant due to its capability of studying protein structures in solution. However, NMR protein structure determination has remained a laborious and costl... G...
Several recent advancements have transformed solution NMR spectroscopy into a competitive, elegant, and eminently viable technique for determining the solution structures of membrane proteins at the level of atomic resolution. Once a good level of cell-based or cell-free expression and purification of ...
It can provide information on protein surface interaction, their complex structure, and their dynamic properties during protein recognition. Several examples are given in this paper. 展开 关键词: NMR spectroscopy Protein–protein interaction Protein structure Sample preparation Structural genomics Target ...
Advances in AI and deep learning such as protein structure predictors indirectly underscore the need for further analyses of protein motions, an area to which NMR can offer unparalleled insight as discussed previously. Within NMR spectroscopy, neural networks have been developed for peak-picking,141 ...