Nanopore sensing is an emerging technology for single-molecule analysis of biomolecules. Based on ionic current blockade derived from electrically drawn analytes under voltage applied across a nanoscale pore, nanopore sensors offer single-molecule resolution and ultrasensitive, label-free, real-time detectio...
In this emerging era of precision medicine, such tools, when coupled with other proteomic and next-generation, single-cell sequencing approaches33,34,35, can help to provide a more complete picture of disease and effective remedies. Methods Cell culture and kinase inhibitor treatment Primary ...
Qubit dsDNA Broad-Range (BR) and High-Sensitivity (HS) Assay Kits—for sequencing samples, genomic DNA samples and routine cloning experiments (Nucleic Acid Quantitation in Solution—Section 8.3) Qubit RNA Assay Kits—for microarray experiments, rea...
After the completed peptide chain leaves the ribosome, a formyl group (–CHO) is removed by a specific enzyme and, in many cases, the methionine at an end of the N-terminal residue is also removed. Many pieces of ribosomes combine with one molecule of mRNA to efficiently synthesize ...
segment of the polypeptide chain that is able to fold autonomously and that can be found in diverse protein architectures (Fig. 18.1A). Recombination ofprotein domainshas led to the development of large multidomain proteins, whose domains evolved to interact and accomplish one or more functions ...
Fatty acid-binding proteins (FABPs), small cytosolic proteins that function in the uptake and utilization of fatty acids, have been extensively studied in higher vertebrates while invertebrates have received little attention despite similar nutritional r
Zn2+ is required for the activity of many mitochondrial proteins, which regulate mitochondrial dynamics, apoptosis and mitophagy. However, it is not unders
the hydroxyl radical cleaves the DNA backbone non-specifically and at each position along the DNA molecule. The hydroxyl radical is generated by allowing the EDTA complex of iron (II) to react with hydrogen peroxide: [Fe (EDTA) ]2-+ H2O2→ [Fe (EDTA) ]1-+ · OH + OH-. In this rea...
The lamins are the major architectural proteins of the animal cell nucleus. Lamins line the inside of the nuclear membrane, where they provide a platform for the binding of proteins and chromatin and confer mechanical stability. They have been implicated
Interestingly, all five M3-type strains MGAS158, 274, 315, 335, and 1313 that were originally used for scl1-gene sequencing [14], plus an additional strain MGAS2079 (not reported) harbor the same scl1.3 allele containing a null mutation that would result in secretion of a truncated Scl...