Nanopore sensing is an emerging technology for single-molecule analysis of biomolecules. Based on ionic current blockade derived from electrically drawn analytes under voltage applied across a nanoscale pore, nanopore sensors offer single-molecule resolution and ultrasensitive, label-free, real-time detectio...
In this emerging era of precision medicine, such tools, when coupled with other proteomic and next-generation, single-cell sequencing approaches33,34,35, can help to provide a more complete picture of disease and effective remedies. Methods Cell culture and kinase inhibitor treatment Primary ...
After the completed peptide chain leaves the ribosome, a formyl group (–CHO) is removed by a specific enzyme and, in many cases, the methionine at an end of the N-terminal residue is also removed. Many pieces of ribosomes combine with one molecule of mRNA to efficiently synthesize ...
High mass accuracy FT-MS/MS would have a different time economy. Because the MS/MS spectrum has to be recorded after the MS and SIM spectra, doublet directed sequencing is highly advisable. Peaks were picked from the raw data files using DTAsupercharge (version 0.94, made available by ...
For example, one apolipoprotein B (apoB) molecule is embedded in each very-low-density lipoprotein (VLDL), intermediate-density lipoprotein (IDL), low-density lipoprotein (LDL), and lipoprotein(a) (Lp[a]) particle and apoB seems to estimate the atherogenic risk more accurately than the ...
Fatty acid-binding proteins (FABPs), small cytosolic proteins that function in the uptake and utilization of fatty acids, have been extensively studied in higher vertebrates while invertebrates have received little attention despite similar nutritional r
Zn2+ is required for the activity of many mitochondrial proteins, which regulate mitochondrial dynamics, apoptosis and mitophagy. However, it is not unders
The lamins are the major architectural proteins of the animal cell nucleus. Lamins line the inside of the nuclear membrane, where they provide a platform for the binding of proteins and chromatin and confer mechanical stability. They have been implicated
tunnelling currents and nanopores have been proposed. Here, we present a review of these approaches, together with the first experimental efforts towards their realization. We discuss their advantages and drawbacks, and present our perspective on the development of single-molecule protein sequencing techn...
A. et al. The emerging landscape of single-molecule protein sequencing technologies. Nat. Methods 18, 604–617 (2021). CAS PubMed PubMed Central Google Scholar Berhanu, S. et al. Sculpting conducting nanopore size and shape through de novo protein design. Preprint at bioRxiv https://doi....