Single Molecule Real Time (SMRT) Sequencing (Fig. 7) is a technology that can sequence single long DNA molecules. A library of circular, single stranded DNA templates is created by ligation of hairpin adapters to both ends of the target double stranded DNA (Fig. 7B) [154,155...
Smith CC, Brown M, Parker WT, et al. Single Molecule Real Time (SMRTTM) sequencing sensitively detects the evolution of polyclonal and compound BCR-ABL mutations in patients who relapse on kinase inhibitor therapy [abstract]. Blood. 2012;120(21): Abstract 917....
Real-time sequencing method for single moleculeThis invention is to describe a method for the determination of a nucleic acid sequence, in which it involves an enzyme, an enzyme complex or plural number of enzymes with more than one enzymatic activity and a set of nucleotide analogs, to ...
reverse transcription polymerase chain reaction (RT-PCR), single-molecule real-time (SMRT) sequencing, and Circular Consensus Sequencing2 (CCS2). For cases #1–#8, the samples were extracted prior to treatment and after treatment failure of the daclatasvir + asunaprevir (...
Single-molecule real-time (SMRT) sequencing (Pacific Biosciences) produces long and unbiased sequences, which enables assembly of complex repeat structures and GC- and AT-rich regions that are often unassembled or highly fragmented in NGS-based draft genomes. We generated ~72× sequencing coverage ...
in which it involves an enzyme, an enzyme complex or plural number of enzymes with more than one enzymatic activity and a set of nucleotide analogs, to achieve high signal readout accuracy in nucleic acid sequencing by making each signal to have a long signaling time span which allows a high...
Single molecule real time (SMRT) full length RNA-sequencing reveals novel and distinct mRNA isoforms in human bone marrow cell subpopulationsHematopoietic cells are continuously replenished from progenitor cells that reside in the bone marrow... AD Mays,MO Schmidt,GT Graham,... - Genes (Basel) ...
Single-molecule long-read sequencing facilitates shrimp transcriptome research 单分子长read测序促进了虾的转录组研究 Abstract Although shrimp are of great economic importance, few full-length shrimp transcriptomes are available. Here, we used Pacific Biosciences single-molecule real-time (SMRT) long-read ...
We present single-molecule, real-time sequencing data obtained from a DNA polymerase performing uninterrupted template-directed synthesis using four distinguishable fluorescently labeled deoxyribonucleoside triphosphates (dNTPs). We detected the temporal order of their enzymatic incorporation into a growing DNA...
This chapter will first outline the principle of this single-molecule, real-time (SMRT) DNA sequencing method, followed by descriptions of its underlying components and typical sequencing run conditions. Two examples are provided which illustrate that, in addition to the DNA sequence, the dynamics ...