& Shah, N. . ( 2011 ) Single molecule Real Time (SMRT ™ ) sequencing sensitively detects polyclonal and compound BCR-ABL in patients who relapse on kinase inhibitor therapy . 53th ASH Annual Meeting and Exposition. Blood (ASH Annual Meeting s) , 118 , 3752 ....
Single-molecule real-time (SMRT) sequencing, developed by Pacific Biosystems, is a third-generation technology based on sequencing-by-synthesis principle. During library preparation, template DNA fragments are provided with hairpin loop adapters on both sides, which make the template circu...
PacBio采用SMRT测序技术,名称取自single molecule real time sequencing,即单分子实时测序。 上图为PacBio的核心测序原理,基本上包括以下几个要点: 制造出一个ZMW孔(Zero-Mod...前端开发概述(简单笔记) 此系列的全部内容的笔记均来自于b站的python高级编程: python 高级编程 (day07Html和CSS~day10jQuery和js库)...
reverse transcription polymerase chain reaction (RT-PCR), single-molecule real-time (SMRT) sequencing, and Circular Consensus Sequencing2 (CCS2). For cases #1–#8, the samples were extracted prior to treatment and after treatment failure of the daclatasvir + asunaprevir (...
Single molecule real time (SMRT) full length RNA-sequencing reveals novel and distinct mRNA isoforms in human bone marrow cell subpopulationsHematopoietic cells are continuously replenished from progenitor cells that reside in the bone marrow... AD Mays,MO Schmidt,GT Graham,... - Genes (Basel) ...
Single molecule, real-time (SMRT) sequencing enables the detection of N6-methyladenine and N4-methylcytosine, two major types of DNA modifications com- prising the bacterial methylome. However, existing SMRT sequencing-based methods for studying bacterial methylomes rely on a population-level consensus ...
Single-molecule long-read sequencing facilitates shrimp transcriptome research 单分子长read测序促进了虾的转录组研究 Abstract Although shrimp are of great economic importance, few full-length shrimp transcriptomes are available. Here, we used Pacific Biosciences single-molecule real-time (SMRT) long-read ...
In one study that combined scRNA-seq with single-cell single-molecule real-time sequencing (SMRT-seq), it was shown that an AML progenitor cell cluster is associated with dysregulation of RP, characterized by the expression of high-level RP genes and exhibiting poor remission [60]. Another ...
Background: The advent of the Single-Molecule Real-time (SMRT) Isoform Sequencing (Iso-Seq) has paved the way to obtain longer full-length transcripts. This method was found to be much superior in identifying full-length splice variants and other post-transcriptional events as compared to the ...
This chapter will first outline the principle of this single-molecule, real-time (SMRT) DNA sequencing method, followed by descriptions of its underlying components and typical sequencing run conditions. Two examples are provided which illustrate that, in addition to the DNA sequence, the dynamics ...