Protein sequencing and the identification of post-translational modifications are key to understanding cellular signalling pathways and metabolic processes in health and disease. Nanopores, that is, nanometre-sized holes in a membrane, were previously pu
The invention provides a device for determining the type of protein in a liquid, the device comprising (a) an immobilized ATP dependent protease based molecular transporter machine configured to guide a protein that is functionalized with labels through a detection area of a detector, (b) said ...
Proteins are major building blocks of life. The protein content of a cell and an organism provides key information for the understanding of biological processes and disease. Despite the importance of protein analysis, only a handful of techniques are ava
SINGLE MOLECULE PROTEIN SEQUENCING 优质文献 相似文献 参考文献 引证文献Single-molecule protein sequencing through fingerprinting: computational assessment Proteins are vital in all biological systems as they constitute the main structural and functional components of cells. Recent advances in mass spectrometr....
Paving the way to single-molecule protein sequencing. Nat. Nanotechnol. 13, 786–796 (2018). ADS PubMed Google Scholar Howorka, S. Building membrane nanopores. Nat. Nanotechnol. 12, 619–630 (2017). ADS CAS PubMed Google Scholar Feng, J. et al. Identification of single nucleotides in...
Fig. 6.Single-molecule real-time sequencing employs a zero-mode waveguide (ZMW) to enable the observation of individual DNA molecules. Within the ZMW, a single DNA polymerase enzyme is fixed at the bottom, utilizing a single DNA molecule as a template. This structure creates a small, illumina...
In summary, key advances have been made toward facilitated translocation of proteins and peptides through narrow protein nanopore sensors for the realization of single-molecule protein sequencing. The development of protein translocation systems has been an exciting and active research area and further im...
Single-molecule fluorescence ISH seqFISH: Sequential FISH MERFISH: Multiplexed error-robust FISH RCA: Rolling-circle amplification RCP: Rolling-circle product FISSEQ: Fluorescent in situ sequencing STARmap: Spatially-resolved transcript amplicon readout mapping ...
Single-molecule long-read sequencing facilitates shrimp transcriptome research 单分子长read测序促进了虾的转录组研究 Abstract Although shrimp are of great economic importance, few full-length shrimp transcriptomes are available. Here, we used Pacific Biosciences single-molecule real-time (SMRT) long-read ...
Single-cell RNA sequencing JEV: Japanese encephalitis virus CNS: Central nervous system DMEM: Dulbecco’s modified Eagle’s medium dpi: Days post infection IHC: Immunohistochemistry FISH: Fluorescence in situ hybridization UMI: Unique molecular identifier ...