The invention provides a device for determining the type of protein in a liquid, the device comprising (a) an immobilized ATP dependent protease based molecular transporter machine configured to guide a protein that is functionalized with labels through a detection area of a detector, (b) said ...
SINGLE MOLECULE PROTEIN SEQUENCING 优质文献 相似文献 参考文献 引证文献Single-molecule protein sequencing through fingerprinting: computational assessment Proteins are vital in all biological systems as they constitute the main structural and functional components of cells. Recent advances in mass spectrometr....
Proteins are major building blocks of life. The protein content of a cell and an organism provides key information for the understanding of biological processes and disease. Despite the importance of protein analysis, only a handful of techniques are ava
Paving the way to single-molecule protein sequencing. Nat. Nanotechnol. 13, 786–796 (2018). ADS PubMed Google Scholar Howorka, S. Building membrane nanopores. Nat. Nanotechnol. 12, 619–630 (2017). ADS CAS PubMed Google Scholar Feng, J. et al. Identification of single nucleotides in...
In summary, key advances have been made toward facilitated translocation of proteins and peptides through narrow protein nanopore sensors for the realization of single-molecule protein sequencing. The development of protein translocation systems has been an exciting and active research area and further im...
Next-generation sequencing NSCLC: Non-small cell lung cancer PanIN: Pancreatic intraepithelial neoplasia PCR: Polymerase chain reaction PD-1: Programmed cell death protein-1 PDA: Pancreatic ductal adenocarcinoma scRNA-seq: Single cell RNA sequencing TCR: T cell receptor TIL: Tumor infiltrat...
Single-molecule long-read sequencing facilitates shrimp transcriptome research 单分子长read测序促进了虾的转录组研究 Abstract Although shrimp are of great economic importance, few full-length shrimp transcriptomes are available. Here, we used Pacific Biosciences single-molecule real-time (SMRT) long-read ...
In contrast with advances in massively parallel DNA sequencing, high-throughput protein analyses are often limited by ensemble measurements, individual analyte purification and hence compromised quality and cost-effectiveness. Single-molecule protein detection using optical methods is limited by the number of...
ddELISA can detect proteins inthe low attomolar range and is up to 25-fold more sensitive than digitalELISA using Single Molecule Arrays (Simoa), the current gold standardtool for ultrasensitive protein detection. Using ddELISA, we measuredthe LINE1/ORF1 protein, a potential cancer biomarker that...
Quality control of single-cell RNA sequencing data was executed with the following criteria: > 200 genes/cell, < 3000 genes/cell, > 3 cells/gene, and < 20% mitochondrion genes. The batch effect of 9 samples was eliminated using IntegrateData of Seurat packages [32]. The...