A single pair of enzymes — O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA) — controls the dynamic cycling of this PTM. Potential mechanisms that enable a single OGT enzyme to recognize hundreds of protein substrates include substrate-specific interactions with the tetratricopeptide repeat (TPR)...
1)10. The reverse reaction is catalyzed by β-N-acetylglucosaminidase or O-GlcNAcase (OGA; Fig. 1)11. Thus, O-GlcNAcylation competes with phosphorylation for the same residues to regulate protein function and a wide spectrum of cellular signaling and biological activities12,13,14,15. O-...
Aberrant protein O-GlcNAcylation may contribute to the development and malignant behavior of many cancers. This modification is controlled by O-linked β-N-acetylglucosamine transferase (OGT) and O-GlcNAcase (OGA). The aim of this study was to determine the expression of O-GlcNAc cycling enzymes ...
Conclusions TG induced the expression of chondrogenic markers in ATDC5 cells, and increased chondrogenic differentiation in vivo. Our results show that o-GlcNAc glycosylation has chondromodulating activity. 展开 关键词: Bone Sclerostin antibody Trabecular microarchitecture Glucocorticoids DOI: 10.1016/j.joca...
O-GlcNAcase PEPCK: Phosphoenolpyruvate carboxykinase PFK: Phosphofructokinase PFK: Phosphofructokinase PTMs: Post-translational modifications PC: Pyruvate carboxylase PK: Pyruvate kinase AKT: Protein kinase B SUMO: Small ubiquitin-like modifier SGLTs: Sodium-dependent glucose transporters TRIF:...
elegans, O-GlcNAcase (oga-1) and O-GlcNAc transferase (ogt-1) homozygous null mutants are viable and appear overtly wild type [18, 19]. However, we observed that oga-1(ok1207) null mutant adults live ~33% longer than the wild-type animals, while ogt- 1(ok1474) null mutants...
Our aim was to investigate whether insulin-induced chondrocyte hypertrophy occurs through a modification in the amount of O-GlcNAc glycosylated proteins and in the expression of the key enzymes of this pathway: O-GlcNAc-transferase (OGT) and O-GlcNAcase (OGA). Furthermore, the authors studied if...
ESM_1 35 Protein Ligand Name Formula Reference Comments 2JIW BEU Ki 6.3 1.95 O-Glcnacase Bt_4395 2JLB UDM Kd 7.3 2.5 Xcc0866 H 2NMO LAT Kd 5.0 1.35 Galectin-3 2OYK 9MR Ki 7.2 1.5 Endoglycoceramidase II 2OYL IDC Ki 8.6 1.8 Endoglycoceramidase II 2OYM MNI Ki 6.8 1.86 End...
Interrogating the role of O-GlcNAc on a single target protein is crucial, yet challenging to perform in cells. Herein, we developed a nanobody-fused split O-GlcNAcase (OGA) as an O-GlcNAc eraser for selective deglycosylation of a target protein in cells. After systematic cellular optimization,...
(e) Effect of Eogt RNAi on O-GlcNAcylation of endogenous Notch. Proteins immunoprecipitated from Kc cell lysates with anti-Notch antibody were detected by anti-O-GlcNAc antibody. 2 NATURE COMMUNICATIONS | 2:583 | DOI: 10.1038/ncomms1591 | www.nature.com/naturecommunications © 2011 Macmillan ...