Prostate cancer is the second most common malignancy in men worldwide and consists of a mixture of tumor and non-tumor cell types. To characterize the prostate cancer tumor microenvironment, we perform single-cell RNA-sequencing on prostate biopsies, prostatectomy specimens, and patient-derived organo...
Pentose phosphate pathway37, Citrate cycle); metabolic alteration is a hallmark of cancer38. The activated TCA cycle pathway is essential for a neoplastic prostate cell to evolve into a malignant tumor cell39and its activations in the center suggests...
We have previously isolated and characterized a new adherent prostate cancer cell line derived from a vertebral metastatic lesion (VCaP) [9]. The cells were characterized for tumorigenicity, doubling time, karyotype, and expression of cytokeratin-18, wild-type androgen receptor, androgen sensitivity, ...
-integrins to strengthen adhesion to collagen type I Eph receptor (Eph) and ephrin signaling can play central roles in prostate cancer and other cancer types. Exposed to ephrin-A1 PC3 prostate cancer cells al... M Yu,J Wang,DJ Muller,... - 《Scientific Reports》 被引量: 0发表: 0年 ...
8.12.9 Prostate cancer Prostate cancer is a slowly progressing type of cancer with cell propagation occurring before its clinical appearance. According to a report published by US National Cancer Institute, an estimated 238,590 individuals were affected by prostate cancer and 29,720 cases of deaths...
However, depending on the type of drug or cancer cell, activation of NF-κB can elicit a pro-death response [6]. Our results indicated that improving the cell death response to 2ME2 and Doc in PC cells depends on stimulating rather than inhibiting NF-κB activity. In contrast to what ...
Like any cancer type, prostate cancer arises when changes in your cells at the genetic level make them grow and divide more than normal. Sometimes, those cells may grow or spread to other nearby parts of your body. They may also spread to other more distant parts of the body. In most ...
Functional role of miRNA dysregulation in prostate cancer The cellular characteristics required for the initiation and progression of cancer have been described and involve a broad set of biological pathways including those that drive cell growth, inhibit cell death, and promote angiogenesis and metastasis...
prostate cancer cell clones (See Methods). We first validated this system through single CRE deletions, where we transiently transfected a set of gRNA targeting the CRE of interest. In accordance with data from our prostate cancer cell clones stably expressing wild-type Cas9 and dCas9-KRAB, ...
we functionally validate our observations and add single-cell resolution. Thereby, we show that tumor progression occurs through transcriptional adaption rather than a selection of pre-existing cancer cell clusters. Moreover, we determine at the single-cell level how inhibition of EZH2 - the top up...