OriginalArticles Liposomalinsulinpromoter–thymidinekinasegenetherapyfollowed byganciclovireffectivelyablateshumanpancreaticcancerinmice JamesX.Wu a ,Shi-HeLiu a ,JohnJ.Nemunaitis b ,F.CharlesBrunicardi a, * a DavidGeffenSchoolofMedicine,UCLA,LosAngeles,CA,USA b MaryCrowleyCancerResearchCenters,Dallas,TX...
Liposomes encapsulating PSA promoter-driven perforin expression vector (pLipo) were constructed for its specific expression in PC. The anti-tumor effect of pLipo was evaluated in vitro using docetaxel-resistant PC 22Rv1 PC cell line, 22Rv1DR, and PC-3 cells in the presence of human ...
In vivo disposition characteristics of plasmid DNA complexed with cationic liposomes J. Drug Target., 3 (1995), pp. 149-157 CrossrefView in ScopusGoogle Scholar 32 O. Thanaketpaisarn, M. Nishikawa, F. Yamashita, M. Hashida Tissue-specific characteristics of in vivo electric gene: transfer ...
Cancer Gene Ther 3: 11–17 PubMed CAS Google Scholar Isaka Y, Akagi Y, Kaneda Y, et al (1998) The HVJ liposome method. Exp Nephrol 6: 144–147 Article PubMed CAS Google Scholar Gaber MH, Wu NZ, Hong K, et al (1996) Thermosensi-tive liposomes: extravasation and release of ...
Examples of other vectors include viruses such as bacteriophages, baculoviruses and retroviruses, DNA viruses, cosmids, plasmids, liposomes and other recombination vectors. The vectors can also contain elements for use in either procaryotic or eucaryotic host systems. One of ordinary skill in the ...
Systemic delivery of cationic liposome-complexed plasmids targeting FAK, resulted in the diminishment of subcutaneous tumor growth beyond the effects observed with liposomes carrying a non-specific shRNA. This diminishment in growth was associated with elevated levels of apoptosis (TUNEL staining), ...
One potential therapy under active investigation is treating tumors with recombinant viral vectors expressing anti-cancer therapeutic proteins. Adenoviral vectors (Ad) have proven to be of enormous utility for a variety of gene therapy applications. Adenovirus-based vectors contain several characteristics th...
Furthermore, to elucidate the advantages of using liposomes as a gene carrier, we screened more than 20 liposome preparations in this study. The 5′ upstream region of PSA gene (PSARPSAP) was conjugated to either the chloramphenicol acetyltransferase (CAT) gene or herpes simplex virus thymidine ...
In the present study, we attempted to take advantage of metallothionein overexpression to overcome cisplatin resistance.Using a virus-free system (liposomes), we sought to express the suicide gene, thymidine kinase (TK), driven by the promoter of the human metallothionein IIa (hMTIIa) gene using...
After inoculation with the tumor, HVJliposomes containing the AFPTK1 plasmid vector were injected into the portal vein via the splenic hilum, followed by GCV treatment. This gene therapy significantly inhibited the growth of tumors in the liver and markedly improved survival. Three injections of the...