Cancer immunotherapies based on reinvigoration of exhausted T cells, such as checkpoint blockade therapies, have provided therapeutic benefit. Nevertheless, many patients still do not achieve durable responses (Thommen and Schumacher, 2018), and it has been proposed that such therapeutic failures can pot...
Human umbilical cord blood-derived CD133+ cells enhance function and repair of the infarcted myocardium. The use of adult stem cells for myocardial tissue repair might be limited in elderly and sick people because their cells are depleted and exhausted. The pr... J Leor,E Guetta,MS Feinberg,...
Several LAG-3-expressing subsets of CD4 T cells have been described. These include Foxp3-dependent Tregs29 and Foxp3-independent, IL-10-producing regulatory Tr1 cells23; Tregs that regulate B cell responses30; and exhausted T cells that co-express LAG-3 and other checkpoint inhibitors, such ...
Unlike the mesonephric kidney, which can add nephrons throughout adult life via long-lived stem cells30, the mammalian metanephric kidney has a finite window during which nephrons are generated. In humans, NPCs are exhausted en masse by 34–36 weeks of gestation, with 60% of nephrons ...
heart field to the heart primordium is exhausted around HH18 (Waldo et al.2001) and correlates with cells expressingIsl1(van den Berg and Moorman2009). Recent experiments have demonstrated that the chicken pharyngeal mesoderm also contributes to the pharyngeal muscles (Tirosh-Finkel et al.2006; ...
Clustering of transcriptomic profiles corresponds to A2B5 and KIAA1549:BRAF status of PA cells.at-SNE plot showing PA single cells colored by six tumors of origin.bt-SNE plot showing PA single cells colored by A2B5 glial progenitor marker status as determined by immunolabeling.ct-SNE plot sho...
[4]. Therefore, SMCs are an attractive target for treatment in regenerative medicine. During embryonic development, endothelial cells in the nascent hollow organs recruit smooth muscle progenitor cells (pSMCs) to be invested in tubular walls [5]. Because of their unexhausted differentiation and ...
Interestingly, IL1β significantly enhanced the self-renewal capacity of Tet2-KO LSKs relative to vehicle-treated Tet2-KO cells in a serial re-plating assay with increased CFU-GMs or CFU-Ms up to the quaternary plating (Fig. 2d). In contrast, WT LSKs were exhausted by the tertiary ...
These “stem-like” cells differentiate into GZMB+PRF1+GNLY+ZNF683+ effector TRM-like T cells (TRMeff), and then into TIGITlowHAVCR2+/lowHLA-DRhighENTPD1+/lowTOX+ TILs with a highly proliferative signature (TRMprol; Figure S2H), before turning into truly exhausted PD-1highHAVCR2high...
For culture experiments, cells were sorted into specific subsets within the lineage marker (glycophorin A [GPA], CD13, CD14, CD19, CD20, CD3, CD8, or CD56)–negative CD34+ population after enrichment of CD34+ cells. Murine staining reagents. The indicated Abs were used for flow cytometric ...