A Recommended Procedure for DNA Extraction from Plant Tissues A Recommended Procedure for DNA Extraction from Plant Tissues
The polymerase chain reaction (PCR) DNA amplification method is a powerful tool for the retrospective analysis of formalin-fixed and paraffin-embedded tissues.'-4 To utilize DNA from paraffin-embedded materials, several steps are required and may include transfers of DNA extracts to another container...
DNA extraction ->PCR->Gel electrophoresis-> PCR cleanup->Sanger seq->seq analysis DNA extraction extract the nuclear & mitochondrial DNA i.e. to obtain a solution of pure DNA in water polymerase chain reaction (PCR) to amplify a specific, small fragment of the genome agarose gel electrophoresi...
Development of a microfluidic module for DNA purification via phenol extraction Purification of Deoxyribonucleic acid (DNA) by organic-aqueous liquid extraction, also called phenol extraction, is a standard technique commonly utilized in biology laboratories. In order to minimize interaction energies, ...
The purpose of this study was to develop a simple procedure for cell lysis and DNA extraction for direct detection of Mycobacterium ulcerans in aquatic insects, gills and intestinal contents of fish, molluscs and human tissue samples using a nested PCR method specific for the insertion sequence IS...
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Although 3 DBS (~ 15 μl of blood) had been used for DNA extraction for LAMP by the PURE method and by Maxwell for nested PCR, there is inherent difference between the 2 methods. The PURE extraction renders about 120 μl of DNA solution of which 30 μl (corresponding to ~ 3...
DNA extractionPCRVibrio parahaemolyticusAfter the first outbreak of Acute Hepatopancreatic Necrosis Disease (AHPND) in China in 2009, this disease has been still considered a globally dangerous disease in shrimp aquaculture industry. Currently, there is no effective method to prevent and treat AHPND. ...
The preparation and use of particulate materials for the removal of proteins from nucleic acid samples by solid-phase extraction procedures are described. The solid-phase extraction procedure is analogous to the classical phenol extraction for DNA purification, with the exception that the phenol is rep...
This paper describes rapid and efficient DNA extraction methods for mature leaves, resting buds and seedling leaves of genera in the family Proteaceae. The procedures combine and modify previously published techniques. The DNA can be digested by restriction endonucleases and is suitable for subsequent ...