There is provided a method of extracting both human specific and tumour specific DNA from dried faecal smears. This method allows the screening of the extracted DNA for genetic mutations or markers associated with specific diseases. In particular this method m be used to test for gastrointestinal ...
Total DNA from tannage, tail skin, scales and the skin treated with salt were successfully extracted with an improved method. To verify the result, four pair of primers, which were universal primers for 12S rRNA gene, identification primers of Chinese alligator Alligator sinensis meat, microsate...
DNA extraction from feces has been complicated by two methodological problems:Most methods are not universally applied and the purity of the DNA is often in adequate to water birds.Previously used methods were found unsuitable for water birds such as Hooded Crane(Grus monacha).In this study we ...
DNA was eluted from the column in two steps by adding 50 μL pre-heated (70 °C) 25 % AE (QIAGEN) and incubating the column for two minutes at 70 °C. This was repeated, to reach a total extraction volume of 100 μL DNA extract. DNA quantification with an Alu assay [13,14] ...
Table 1. Genomic DNA extraction protocol StepProcedure 1 Dilute 500 μL of milk sample with 500 μL of sterilized saline solution (NaCl 0.9%) and centrifuge for 15 min at 600 × g at 4°C; discard the supernatant. Repeat the step once. 2 Add 300 μL of lysis buffer1 and 200 μL...
Vincent Phalip ET AL: " Application of a yeast method for DNA extraction associated with database interrogations for the characterization of various ... V Phalip,D Hatsch,JM Jeltsch - 《Biotechnology Letters》 被引量: 24发表: 2004年 MUBII-TB-DB: a database of mutations associated with an...
deer ad the control,further confirmed the reliability of the method.[Conclusion] The method requires no proteinase K in the process of extraction,and the extracted DNA can be used for PCR amplification directly without the purification of DNA purification kit,thus,the cost for the test is very ...
Automated systems have been increasingly utilized for DNA extraction by many forensic laboratories to handle growing numbers of forensic casework samples while minimizing the risk of human errors and assuring high reproducibility. The step towards automation however is not easy: The automated extraction m...
基因组DNA提取 1. Study on the method and conditions for spinach genomic DNA extraction; 菠菜基因组DNA提取方法与条件的研究 2. Objective To study the genomic DNA extraction from Rhizoma Coptidus and optimization of RAPD reaction system. 方法采用目前较为流行的苯酚法、CTAB法、高盐低pH值法,通过...
(1–5). However, this technique presents persistent problems of reproducibility that have been attributed to a high sensitivity to subtle procedure changes and to the quality of the DNA used as template (6–8). For this reason, other techniques that are more time and budget demanding, such ...