The main aim of this study was to compare the different methods of DNA extraction from snap-frozen tissues (SFTs). The samples were submitted to a six different DNA extraction methods -four manual and two using commercial DNA isolation kits. All the retrieval methods were compared with each ...
of DNA extracted by SDS method was the most amoung the four methods;when the quality of Staphylococcus Aureus' DNA diluted to 1.975×10-4 pg,SDS method can still detect the extractives by PCR. SDS method was stable and economic and was an ideal extraction of Staphylococcus Aureus'DNA. ...
组DNA提取方法都具有操作简单、耗时短、利于快速检测的优点。 豆粉;基因组DNA;提取;聚合酶链式反应 S565.1A Comparison of Different Extraction Methods of Genomic DNA from Soybean Powder Xu Weili Ma YingDu MingWang JiaqiLi Qiming 四川省应用基础研究(2009JY0101);哈尔滨工业大学优秀青年教师培养计划(HITQNJS....
DNA also could be extracted from silica gel-dried sample though the amount was low (51.2?滋g/g) and no clear and perfect polymorphiepatterns was found. 展开 关键词: Betula Luminifera Preserved methods DNA extraction Polymerase chain reaction ...
Obtaining a sufficient quantity of high-quality, intact RNA is the first crucial step in its study by RNA sequencing on next-generation sequencing platforms or quantitative PCR. Different RNA extraction methods or commercial kits vary in yield and in the quality and integrity of the RNA obtained,...
提活性污泥总DNA效果最好,以该方法提取的总DNA为模板,PCR扩增16SrDNA,可扩增出分子量为1.5Kh 左右的DNA产物。本文为研究乳品废水活性污泥总DNA的提取提供了一种简便、快速的方法。 关键词:乳品废水;活性污泥;DNA;提取;PCR 中图分类号:X703 文献标识码:A ComparisonofDifferentDNAExtractionMethods fromDairyWastewater...
Comparison of two methods of high-molecular-weight DNA isolation from human leucocytes. Among the different methods for isolation of high-molecular-weight DNA from leucocytes, two of them were retained as being the most interesting and were compared. This choice was based on three criteria: the ...
地锦草不同提取方法对抑菌效果的影响The comparision among the several different extraction methods in the research of the effective components of Humifusa Eu... 汪成竹,王春枝 - 《文章详细信息》 被引量: 0发表: 0年 不同方法对枣叶片总DNA提取效果的影响 以串杆枣的成熟鲜叶为试材,分别采用-20℃,-...
In this study, we compared different cell lysis methods and evaluated cell lysis efficiency through microscopic visualization. In addition, we compared three commercially available RNA extraction methods and determined RNA yield, RNA purity and RNA integrity. In addition, we studied the effect of stora...
Our ongoing study is aiming to test the impact of three different age of faeces samples and two DNA extraction methods on the genotyping success of Sumatran tiger (Panthera tigris sumatrae). Samples from zoos in Germany are separated into three different part (fresh, 1 week and 1 month). ...