Therefore, the pro-apoptotic proteins Bax and Bak were silenced in the human corneal endothelial cell line (HCEC-12) by transfection with AccellsiRNA without any adverse effects on cell viability. When apoptosis was induced, e.g., etoposide, the caspase-3 activity and Ann...
Bcl-2-family proteins are central regulators of cell life and death. At least three major classes of Bcl-2-family proteins have been delineated, including proapoptotic proteins that contain several conserved regions of sequence similarity (termed ‘multidomain’). In mammals, the multidomain protein...
Background: During apoptosis Bax/Bak release differently sized proteins out of the mitochondria. Results: The size of Bax/Bak pores depends on protein concentration. Conclusion: Bax/Bak form stable toroidal pores tunable in size. Significance: Pore size-tuning constitutes a new level for the regula...
Given that PCAF and its binding partner transcriptional adaptor protein 3 (ADA3) were recently shown to regulate the intrinsic (mitochondrial) pathway to apoptosis via epigenetic regulation of phosphofurin acidic cluster sorting proteins 1 and 2 (PACS1, PACS2), we analyzed PCAF, ADA3, and PACS...
Objective: To study the roles of different truncated hepatitis C virus (HCV) core proteins (CORE) in the pathogenesis of HCV persistent infection and hepatocellular carcinoma (HCC) and to assess intracellular localization in transiently transfected cells. Methods: Seven truncated CORE-GFP (green fluore...
To further elucidate the pathway by which AAL induced this apoptosis, western blot analysis was performed to detect the levels of several proteins pertaining to this pathway. Poly [ADP-ribose] polymerase-1 [PARP-1] is a strand break sensor involved in DNA repair. The cleavage of PARP-1 resul...
However, activation of pro-apoptotic proteins did not lead to induction of apoptosis. These observations suggest that autophagy governs the downstream pro-apoptotic effectors to execute growth arrest in BCa cells. Induction of autophagic signalling by NA is dependent on LC3B activation. Next, we ...
In the intracellular death program, hetero- and homodimerization of different anti- and pro-apoptotic Bcl-2-related proteins are critical in the determinat... Sheau,Yu,Hsu,... - 《Proceedings of the National Academy of Sciences of the United States of America》 被引量: 634发表: 1997年 Mod...
Expression analysis of apoptosis-associated proteins in the HCB289 cell line after 24 h of exposure with rhTRAIL ligand, WT HSV-1 and in combination. (A) and (B) detection and quantification of proteins after treatment. Results are expressed as mean percentage ± standard deviation of rela...
Protein disulfide isomerase (PDI) family proteins are classified as enzymatic chaperones for reconstructing misfolded proteins. Previous studies have shown that several PDI members possess potential proapoptotic functions. However, the detailed molecular mechanisms of PDI-mediated apoptosis are not completely ...