Essential role of the iron-sulfur cluster binding domain of the primase regulatory subunit Pri2 in DNA replication initiation. Protein Cell 2015, 6, 194-210.L. Liu, M. Huang, Essential role of the iron-sulfur cluster binding domain of the primase regulatory subunit Pri2 in DNA replication ...
Primases play a crucial role in the initiation of DNA synthesis during replication by de novo synthesis of short RNA or DNA “primers.” In recent years, evidence has accumulated which expands the essential roles of primases to include, not only the initiation of replication but also other cr...
Role of DNA polymerase alpha and DNA primase in simian virus 40 DNA replication in vitro. The role of DNA polymerase alpha (pol alpha) and DNA primase has been investigated in the simian virus 40 (SV40) DNA replication system in vitro. Removal o... Y,Murakami,C,... - 《Proceedings of...
DNA polymerase I, DNA ligase, and Topoisomerase I each catalyze the formation of phosphodiester bonds that are vital (directly or indirectly) to replication of DNA. What is the action of each of these enzymes in DNA replication...
Primase activity is essential for optimal checkpoint signaling at stalled replication forks (25-28) and possibly in rescuing stalled replication fork progression (29), but its interaction partners are not known.To better understand the role of human primase in these pathways, it will be vital to ...
Biological Role in the Cell Initiation of Chromosome Replication DNA polα primase has been regarded as the principal DNA polymerase involved in the initiation of chromosomal DNA synthesis. Reconstituted replication of an SV40 origin-containing plasmid by purified replication factors confirms that the pol...
A single essential gene, PRI2, encodes the large subunit of DNA primase in Saccharomyces cerevisiae. DNA primase activity of the yeast DNA polymerase-primase complex is related to two polypeptides, p58 and p48. The reciprocal role of these protein species ... M Foiani,C Santocanale,P Pleva...
PRIM1 mapping at chromosome 12q13 is the smallest subunit within a polymerase α (Pol α) heterotetrameric complex, which plays a crucial role in DNA replication by facilitating catalytic function and synthesizing RNA primers as starting point for the replication of the leading and lagging strand...
The latter enzyme extends the short RNA primer with DNA, prior to transfer of the hybrid nucleic acid to the replicative DNA polymerase. Notably, DNA Pol α exhibits a strong preference for extending a RNA primer on a DNA template. The thumb domain of Pol α plays a key role in ...
Additionally, the CST complex removes G-quadruplex DNA structures that form within G-rich regions of the genome, especially at telomeres16. Still, new functions for the CST complex are being uncovered such as its role in replication stress17. Notably, numerous mutations within the CST complex ...