DNA replication is a cellular process that is replication for the duplication of DNA content in cells. A number of enzymes and proteins are involved in the replication. During replication, each DNA strand of the double-stranded DNA (dsDNA) serves as the template strand for the synthesis of ...
Essential role of the iron-sulfur cluster binding domain of the primase regulatory subunit Pri2 in DNA replication initiation. Protein Cell. 2015; 6:194-210. [PubMed: 25645023]L. Liu, M. Huang, Essential role of the iron-sulfur cluster binding domain of the primase regulatory subunit Pri2 ...
PRIM2, encoding a subunit of primase involved in DNA replication and transcription, is expressed in the placenta and is crucial for mammalian development and growth. Its role in placental function is not well understood. Recently, PRIM2 was reported as imprinted in human white blood cells (WBC)...
DNA Replication: DNA replication is the process of making two copies of DNA from one parent strand. The process is complex, especially in eukaryotes that store their DNA in the nucleus. Many enzymes are involved in DNA replication, such as DNA polymerase, primase, helicase, topoisomerase and mo...
In DNA replication, the first two bases are always RNA, and are synthesized by another enzyme called primase. WikiMatrix DNA polymerase cannot add primers, and therefore, needs primase to add the primer de novo. WikiMatrix In eukaryotic replication, the primase forms a complex with Pol α...
DNA primase serves as a key target for regulation of DNA replication initiation, telomere maintenance, and response to DNA damage or fork stalling, in part through its physical interactions with other proteins involved in DNA replication and in checkpoint signaling (19). Primase interacts physically ...
In baker׳s yeast, the genes are named pril (small primase subunit), pri2 (large primase subunit), pol2 (subunit B), and poll (DNA polymerase alpha). Mutations within the two primase and single DNA polymerase genes lead to phenotypes consistent with other DNA replication mutants. For ...
For DNA replication in vivo, DNA primase uses a complementary single-stranded DNA template to synthesize RNA primers ranging from 4 to 20 nucleotides in length, which are then elongated by DNA polymerase. Here, we report that, in the presence of double-stranded DNA, the thermophilic DNA ...
Domain mapping of Escherichia coli RecQ defines the roles of conserved N- and C-terminal regions in the RecQ family. RecQ DNA helicases function in DNA replication, recombination and repair. Although the precise cellular roles played by this family of enzymes remain elusi... A Douglas,Bernstein...
Transcription-replication conflicts (TRCs), especially Head-On TRCs (HO-TRCs) can introduce R-loops and DNA damage, however, the underlying mechanisms are still largely unclear. We previously identified a chloroplast-localized RNase H1 protein AtRNH1C that can remove R-loops and relax HO-TRCs for...