Despite extensive studies on DNA replication, the exchange mechanisms of DNA polymerase during replication remain unclear. Existing models propose that this exchange is facilitated by protein partners like heli
The leading strand is the simplest to replicate. Once the DNA strands have been separated, a short piece of RNA called a primer binds to the 3' end of the strand. The primer always binds as the starting point for replication. Primers are generated by the enzyme DNA primase. Why do Okaz...
What is the directionality of both DNA strands in the replication fork? What is a template in DNA replication? What does RNA primase do in DNA replication? What type of replication is DNA replication? What enzyme produces a new DNA strand during DNA replication?
DNA replication is the process of duplicating a cell's entire genome so as to have a complete genome to passed on to each of two daughter cells that will arise from a single mother cell during mitosis. This process always occurs moving from the 5' end of a DNA strand to...
Particularly, we demonstrate redundancy of the Pol α-primase DNA polymerase activity in replication and show that Pol α-primase and the lagging-strand Pol δ can be re-used within the replisome to support the synthesis of large numbers of Okazaki fragments. This unexpected malleability of the ...
Among them, DNA polymerase a/primase, replication protein A (RPA), and topoisomerase I (topo I) are believed to participate in DNA replication at a very early stage =-=(19, 21, 37, 40, 41, 51, 57, 59, 63, 64, 65, 67)-=-. Topo I is a critical enzyme needed to release the...
This pathway is associated with the core of the DNA replication machinery comprising CDC45, the replicative MCM2-7 hexamer, GINS (altogether forming the CMG complex), primase–polymerase (POLε, -α, and -δ) complex, and additional fork protection factors such as AND-1, CLASPIN (CLSPN), ...
histone H1, which may be important for chromosome condensation that is required during DNA replication. Histone H1 is also a substrate for cyclin B-CDK1 complexes. Finally, the cyclin A-dependent kinases regulate initiation of DNA replication by phosphorylating the DNA polymerase alpha primase [20]...
5B). The changing trend of crucial enzyme genes in DNA replication of archaea and eukaryotes were consistent with the changing trend of their abundance, but the situation in bacteria was not. For bacterial DNA replication, only the interaction of initiator protein DnaA with replication origin (...
Answer to: The enzyme DNA ___ reads one strand of the original DNA and uses it as a blueprint to "write" a new strand during replication. By...