RNases are a broad class of enzymes that are specific for cleaving RNA molecules. RNases are commonly used in molecular biology laboratories during the purification of DNA and proteins. Different sub-classes of RNase are present, such as RNase A, RNase H, RNase P, RNase L, RNase III, ...
A role for DNA primase in coupling DNA replication to DNA damage response. EMBO J. 16 : 639-650.Marini F, Pellicioli A, Paciotti V, Lucchini G, Plevani P, Stern D F, Foiani M. A role for DNA primase in coupling DNA replication to DNA damage response. EMBO J. 1997; 16 :639–...
What is the function of primase in DNA replication? Explain the role of DNA polymerases in replication. Describe how DNA methylation can activate gene expression. What role do single-strand binding proteins play in DNA replication? What is the role of DNA and RNA in protein formation?
A DNA Polymerase-α·Primase Cofactor with Homology to Replication Protein A-32 Regulates DNA Replication in Mammalian Cells α-Accessory factor (AAF) stimulates the activity of DNA polymerase-α·primase, the only enzyme known to initiate DNA replication in eukaryotic cells (Gou... Darren,E.,Cas...
Such a model is reminiscent of the role of SSB in the hand-off of RNA primers from primase to the DNA polymerase III holoenzyme during bacterial DNA replication. In this mechanism, primase binds to an RNA primer only when it is simultaneously bound to SSB; the primer/template is handed ...
However, since in vitro experiments demonstrated that primers for lambda DNA replication are produced by the dnaG gene product (DnaG primase) and subsequent in vivo studies indicated that oop RNA is an antisense RNA for the lambda cII gene expression, the above-mentioned hypothesis has fallen ...
In eukaryotes, DNA polymerases (Pols)3 α, δ, and ϵ jointly support DNA replication, and their overall roles in this process have been defined (1). The initiation of DNA replication is catalyzed by the Pol α-primase complex, composed of the Pol α complex (p180-p70) associated with ...
The operon that encodes the sigma subunit of RNA polymerase also encodes ribosomal protein S21 and DNA primase in E. coli K12. The sigma subunit of E. coli RNA polymerase is encoded by the rpoD gene. Within the sequence upstream from rpoD, we have identified the structural genes rp... ZF...
With the ever-expanding availability of DNA sequence data, it has become increasingly clear that genes and genomes can evolve at very different rates. Organelle genomes, in particular, provide dramatic examples of variation in nucleotide substitution rat
Comparison to PDB enabled annotation of three families with no PFAM and KEGG annotations as having functions related to the DNA replication machinery (Additional file1: Table S4). We were interested to find that this ubiquitous function is performed by specific protein families in Crenarchaeota, poss...