Marini F , Pelliccioli A , Paciotti V , Lucchini G , Plevani P , Stern DF and Foiani M ( 1997 ) A role for DNA primase in coupling DNA replication to DNA damage response . EMBO J , 16 , 639 – 650 .Marini F, Pellicioli A, Paciotti V, Lu...
When DNA replication is inhibited during the synthesis (S) phase of the cell cycle, a signaling pathway (checkpoint) is activated that serves to prevent mitosis from initiating before completion of replication. This replication checkpoint acts by down-regulating the activity of the mitotic inducer cd...
Transcription-replication conflicts (TRCs), especially Head-On TRCs (HO-TRCs) can introduce R-loops and DNA damage, however, the underlying mechanisms are still largely unclear. We previously identified a chloroplast-localized RNase H1 protein AtRNH1C that can remove R-loops and relax HO-TRCs for...
Single-stranded DNA-binding protein complex from Helicobacter pylori suggests an ssDNA-binding surface. Single-stranded DNA-binding protein (SSB) plays an important role in DNA replication, recombination, and repair. SSB consists of an N-terminal single-stran... KW Chan,YJ Lee,CH Wang,... 被...
Prior to replication, the DNA double helix must be untwisted by which type of enzyme? (a) Topoisomerase (b) Ligase (c) Polymerase (d) Helicase (e) Primase. DNA Replication: DNA replication occurs in the regions of...
DNA Polymerase: Structure and Function Robert D.Kuchta,GudrunStengel, inBiochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2010 Primaseis a particularly intriguing chemotherapeutic target due to its essential role inDNA replication, and a number of inhibitors have been synthesized (Fig. ...
近日,来自美国洛克菲勒大学的Titia de Lange和Joseph T. P. Yeeles研究团队在Nature上发表题为CST–polymerase α-primase solves a second telomere end-replication problem的文章,发现第二个末端复制问题,在体外重构的DNA复制系统中,后随链DNA复制...
The Mpox pandemic, caused by the Mpox virus (or monkeypox virus, MPXV), has gained global attention. The D5 protein, a putative helicase-primase found in MPXV, plays a vital role in viral replication and genome uncoating. Here we determined multiple cryo
PRIM1 mapping at chromosome 12q13 is the smallest subunit within a polymerase α (Pol α) heterotetrameric complex, which plays a crucial role in DNA replication by facilitating catalytic function and synthesizing RNA primers as starting point for the replication of the leading and lagging strand...
Biological Role in the Cell Initiation of Chromosome Replication DNA polα primase has been regarded as the principal DNA polymerase involved in the initiation of chromosomal DNA synthesis. Reconstituted replication of an SV40 origin-containing plasmid by purified replication factors confirms that the pol...