DNA replication is the process of making two copies of DNA from one parent strand. The process is complex, especially in eukaryotes that store their DNA in the nucleus. Many enzymes are involved in DNA replication, such as DNA polymerase, primase, helicase, topoisomerase and more. ...
The conserved C-terminal sequence of PriL (PriLCTD) is an iron–sulfur cluster domain with an important functional role in RNA primer synthesis (7,8). In eukaryotes the primase is found tightly assoc...Lao-Sirieix,S.H., Pellegrini,L. and Bell,S.D. (2005) The promiscuous primase. ...
Which of the following enzymes involved in DNA replication is unique to eukaryotes? a. Helicase b. DNA polymerase c. Ligase d. Telomerase What type of enzyme is DNA polymerase? DNA polymerase I, DNA ligase, and Topoisomerase ...
It is during the elongation phase of DNA replication that primase plays a key role in establishing the frequency of Okazaki fragment initiation. Leading and lagging strand DNA synthesis must be coordinated. In isolation, E. coli primase has the lowest catalytic efficiency of any known polymerase. ...
T7 gp4 protein is central to the replication machinery, it is composed of a zinc-binding domain (ZBD) that recognizes ssDNA template, an RNA polymerization domain (RPD) that adds ribonucleotide, and a helicase domain that unwinds double-stranded DNA11. Most eukaryotes own a homolog of the T...
Small tandem duplications of DNA occur frequently in the human genome and are implicated in the aetiology of certain human cancers. Recent studies have suggested that DNA double-strand breaks are causal to this mutational class, but the underlying mechan
We studied the evolutionary relationships of different primase–helicase bifunctional proteins, found mostly in viruses, virophages, plasmids, and org
Structure of a DNA polymerase alpha-primase domain that docks on the SV40 helicase and activates the viral primosome. DNA polymerase alpha-primase (pol-prim) plays a central role in DNA replication in higher eukaryotes, initiating synthesis on both leading and lagging stra... H Huang,BE Weiner...
helicases are non-orthologous between these two primary divisions of cellular life. In contrast, several other components of the DNA replication machinery, such as the DNA ligases, PCNA, clamp-loader ATPases, Topoisomerase Ia and RNAse HII, are orthologous between archaeo-eukaryotes and bacteria ...
Although direct physical and functional interactions between helicase and primase have been known in many prokaryotic and viral systems, potential interactions between helicase and primase have not been explored in eukaryotes. Using purified Mcm and DNA primase complexes, a direct physical interaction is ...