Keywords: Xenopus laevis,hepatocytes,primary culture,perfusion,environmental estrogen非洲爪蟾,肝细胞,原代培养,灌注,环境雌激素 Full-Text Cite this paper Add to My Lib Abstract: Xenopus laevis is a good model to study endocrine disrupters, and the hepatocytes are widely used for assaying estrogenic ...
Bagnard. Protocol for the primary culture of cortical neurons. In: New Methods for Culturing Cells from Nervous Tissues, Vol. 1, edited by P. Poindron, P. Piguet, and E. Forster. Basel: Karger, 2005, pp. 12–22.Gonthier, B., C. Nasarre, T. Rudiger, and D. Bagnard. Protocol for...
was used to demonstrate that cultured cells are cardiomyocytes.Results Beating myocardial cells were clearly observed after 48 hours of culture and 96.7% of cultured cells were live myocardial cells.Conclusion The protocol for isolation and cultivation of myocardial cells from neonatal mice are optimized...
An Optimized Protocol for Culture of Cardiomyocyte from Neonatal Rat Primary culture of cardiomyocytes has been widely used as a valuable tool for pharmacological and toxicological studies. However, the fact that heart is a ... J Fu,G Jie,R Pi,... - 《Cytotechnology》 被引量: 175发表: 2005...
Cell culture and the eukaryotic cells used in biotechnology W T.Godbey, inBiotechnology and its Applications (Second Edition), 2022 7.2.1Primary cells Cells that have been taken directly from a body or a tissue are known asprimary cells, and they can be obtained by biopsy, surgery, or au...
Background: Adipose tissue (AT) is a rich source of mesenchymal stem cells (MSCs), however, there is no standardized protocol for stem cell isolation and culture. This leads to inconsistency of the results and limits the comparison of the data from different laboratories. Our aim was to ...
A step-by-step protocol describing device platform operation can be found at Protocols.io87. Following processing with the minced digestion device, tubing was connected from the outlet of the minced digestion device to the inlet of the integrated dissociation and filtration device. If recirculation ...
Here, we define a simple, non-expensive and reproducible serum-free cell culture protocol for establishment and propagation of primary paediatric brain tumour cultures as adherent monolayers. The success rates for establishment of primary cultures (including medulloblastomas, atypical rhabdoid tumour, ...
For more than 40 years primary hepatocytes culture technique has been utilized extensively for assessing effects of drug on metabolizing enzymes (especially cytochromes P450), drug metabolism, drug-drug interactions, and the mechanisms of cytotoxicity and genotoxicity. Human derived primary hepatocytes reser...
Humanized model systems that enable robust culture of primary human intestinal cells with bacteria facilitate evaluation of potential treatments. Here, we describe a protocol that can be used to coculture a primary human colon monolayer with aerotolerant bacteria. Primary human colon cells maintained ...