Astrocyte functionC6 cellsImmortalized astrocytesThe understanding of the physiology of astrocytes and their role in brain function progresses continuously. Primary astrocyte culture is an alternative method to study these cells in an isolated system: in their physiologic and pathologic states. Cell lines ...
Bio-protocol 相似文献A Novel Serum Free Primary Astrocyte Culture Method that Mimic Quiescent Astrocyte Phenotype Primary astrocyte cultures have been used for decades to study astrocyte functions in health and disease. The current primary astrocyte cultures are mostly... J Prah,A Winters,K Chaudhari...
Animal studies were conducted following the animal study protocol ASP K117-LMB-17 approved by the NIDDK Animal Care and Use committee chaired by Dr. Constance Noguchi. Conventional primary astrocyte culture Primary astrocyte cultures were prepared from cerebral cortices of P2-P5 C57BL/6J mice. ...
Correction to: A primary neural cell culture model to study neuron, astrocyte, and microglia interactions in neuroinflammation N Goshi,RK Morgan,PJ Lein,... - 《Journal of Neuroinflammation》 被引量: 0发表: 2022年 Human mesenchymal stem cell transplantation extends survival, improves motor ...
loaded into a well of a 96 well PLATE. Pooled library amplification for transcriptome expression (PLATE-Seq)55was then performed on the 96 well plate and the resulting data analysis was done as described above. U87 and Astrocyte co-culture PLATE-Seq was conducted the same way as described ...
Effect of Conversion Cocktail on Astrocyte and Neuronal Status in the Primary Hippocampal Culture of 5xFAD Mice with Angiotensin-Converting Enzyme 2 Inhibition doi:10.1134/S0022093024060310Alzheimer’s disease5xFAD miceangiotensin-converting enzyme 2chemical conversion cocktailsMLN-4760Neurodegenerative diseases ...
Effects of interleukin 1β, H2O2 and antioxidants on p38 kinase phosphorylation in primary astrocyte culture: . Free Free Radical Biology and Aging Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104Show moreShow less...
In mock-infected qHA-sp cultures (a), all cells staining for nuclear DAPI also expressed GFAP, indicating a homogenous astrocyte culture (1208 cells counted); in mock-infected qHA-hp cultures (e), 99.4% of cells expressed GFAP (953 cells counted). VZV-infected qHA-sps expressed GFAP (b,...
RESULTS KCOs Facilitated Glutamate Uptake in Rat Primary Astrocyte Cultures in a Concentration-Dependent Manner Astrocytes in culture exhibited a high-affinity glutamate uptake after 48 h of incubation in DMEM with Vmax calculated to 10.6670.06 pM mgÀ1 minÀ1 (Figure 1a). As shown in Figure ...
We also note that we did not include mitotic inhibitors in our cell culture due to concerns about alteration of cellular response due to partial toxicity. Lack of mitotic inhibitors can result in astrocyte contamination of neuronal cultures despite careful visual inspection90,91. It is thus ...