GABA T activity was enhanced and when GABA was added to the culture medium, an induction of this enzyme was demonstrated. GABA T from glia did not differ greatly from the synaptosomal or perikaryal enzyme, and AOAA inhibits its activity as well as that of GABA T from the other cellular ...
星形胶质细胞条件培养液/抗氧化作用5) Flat astrocyte 原浆型星形胶质细胞 1. Culture of processbearing and flat astrocytes in vitro and the proliferating effect of CNTF on them; 纤维型和原浆型星形胶质细胞的分离培养及睫状神经营养因子对其增殖的影响6) primarily cultured kidney cell 原代培养肾细胞补充...
Aspects of this work are reviewed in this article. Such work has already shown that astrocytes in primary culture exhibit the basic electrophy 展开 DOI: 10.1007/BF00734994 被引量: 233 年份: 1983 收藏 引用 批量引用 报错 分享 求助全文 通过文献互助平台发起求助,成功后即可免费获取论文全文。 请先...
The effects of natriuretic peptides on the bradykinin signaling in primary culture of neurons and astrocytes Blockage of cerebral arteries leads to stroke and subsequent development of cerebral edema. In vivo experiments in a mouse model of stroke showed that i.v... K Špiranec,M Dobrivojevi...
Respiration and cell volume of cerebral astrocytes from primary culture were measured in media of various osmolarities. Respiration was measured in 3T3 fibroblasts under similar conditions. Uncoupled respiration and respiration independent of oxidative phosphorylation were obtained by adding dinitrophenol and ...
Here, we report a practical method to culture primary marmoset neurons and astrocytes from brains of medically euthanized postnatal day 0 (P0) marmoset newborns that yield highly pure primary neuron and astrocyte cultures. Primary marmoset neuron and astrocyte cultures can be generated reliably to ...
Cells grown in an atmosphere of 5% CO2/95% humidified air at 37 °C were used after 10–12 days in culture, at which time they consisted of confluent glial cells, which stained positively for glial fibrillary acidic protein (17). Determination of NO Concentration NO production in culture ...
This suggests that soluble factors from neurons protect microglia and astrocytes to become inflammatory reactive. In conclusion we have developed a well characterized culture model system enriched in neurons, taken from newborn rats and cultured in defined media. The neurons express different neuronal ...
High extracellular potassium concentrations stimulate oxidative metabolism in a glutamatergic neuronal culture and glycolysis in cultured astrocytes but ha... Rates of deoxyglucose accumulation and of CO 2 production from [U- 14 C]glucose, or from [U- 14 C]lactate or [2- 14 C]pyruvate (as a...
Cells were incubated for 24 h with a mix (1:1 in volume) of their own culture medium and of the astrocyte-conditioned medium. The primary astrocytes and the MPS VII and CHP 212 cell lines were thus incubated with either 6 (control medium) or 1000 units (transduced culture medium) of ...