GABA T activity was enhanced and when GABA was added to the culture medium, an induction of this enzyme was demonstrated. GABA T from glia did not differ greatly from the synaptosomal or perikaryal enzyme, and AOAA inhibits its activity as well as that of GABA T from the other cellular ...
Many central astrocytic functions in metabolism, amino acid neurotransmission and calcium signaling were discovered using this tissue culture preparation and most of these observations were subsequently found in vivo. Nevertheless, primary cultures of astrocytes are an in vitro model that does not fully ...
RESULTS KCOs Facilitated Glutamate Uptake in Rat Primary Astrocyte Cultures in a Concentration-Dependent Manner Astrocytes in culture exhibited a high-affinity glutamate uptake after 48 h of incubation in DMEM with Vmax calculated to 10.6670.06 pM mgÀ1 minÀ1 (Figure 1a). As shown in Figure ...
Such work has already shown that astrocytes in primary culture exhibit the basic electrophysiological characteristics which had been the only functional property well established for these cells in situ. Further studies of the electrophysiological properties of these cells, which can be correlated with ...
Using radiolabelled markers of the intracellular space, we found that astrocytes in primary monolayer culture prepared from neonatal rat cerebral cortex sw... HK Kimelberg,MV Frangakis 被引量: 57发表: 1986年 Disparate effects of serum on basal and evoked NFAT activity in primary astrocyte culture...
The glycogen content of primary cultured astrocytes was approximately doubled by incubation with 1 m M L-glutamate or L-aspartate. Other amino acids and e... RA Swanson,ACH Yu,PH Chan,... - 《Journal of Neurochemistry》 被引量: 240发表: 1990年 The isolation and long-term culture of nor...
This suggests that soluble factors from neurons protect microglia and astrocytes to become inflammatory reactive. In conclusion we have developed a well characterized culture model system enriched in neurons, taken from newborn rats and cultured in defined media. The neurons express different neuronal ...
The data obtained in this study suggest that CPFX-induced DNA damage might be related to oxidative stress and should be considered for further mechanistic studies of central nervous system toxicity of CPFX. 展开 关键词: Ciprofloxacin Central nervous system Primary culture of astrocytes DNA damage ...
Here, we report a practical method to culture primary marmoset neurons and astrocytes from brains of medically euthanized postnatal day 0 (P0) marmoset newborns that yield highly pure primary neuron and astrocyte cultures. Primary marmoset neuron and astrocyte cultures can be generated reliably to ...
星形胶质细胞条件培养液/抗氧化作用5) Flat astrocyte 原浆型星形胶质细胞 1. Culture of processbearing and flat astrocytes in vitro and the proliferating effect of CNTF on them; 纤维型和原浆型星形胶质细胞的分离培养及睫状神经营养因子对其增殖的影响6) primarily cultured kidney cell 原代培养肾细胞补充...