The neuron-astrocyte ratio was monitored for up to 3weeks after treating the cultures with 0, 1, and 5M of cytosine arabinoside (Ara-C) at DIV 2. Subsequently, from postnatal transgenic (TG) mouse expressing ChR2 on astrocytes, hippocampi were cultured on the microelectrode array (MEA) with...
Here, we aim to validate a previously developed microfluidic system for an astrocyte-neuron cell culture platform, in which astrocytes have been genetically modified to overexpress either a human wild-type (WT) or a mutated form of the super oxide dismutase enzyme 1 (SOD1). Cortical neural ...
Co-culture Cell And Tissue Culture Decapitation Animal Euthanization FACS Cell Proliferation Assay ELISA Antibodies Protein Experimental Models Kits Rattus norvegicus Microglia Astrocytes Neuroinflammation Techniques Co-culture Cell And Tissue Culture Decapitation ...
In order to address whether transfer of PrPSc could occur from neurons to astrocytes, we set up co-culture experiments between the chronically prion-infected donor neuronal cell line ScCAD and naïve acceptor cerebel- lar astrocytes. ScCAD was chosen as a "neuron" donor instead of primary ...
Correction to: A primary neural cell culture model to study neuron, astrocyte, and microglia interactions in neuroinflammation N Goshi,RK Morgan,PJ Lein,... - 《Journal of Neuroinflammation》 被引量: 0发表: 2022年 Human mesenchymal stem cell transplantation extends survival, improves motor ...
New insights on astrocyte ion channels: critical for homeostasis and neuron-glia signaling. J Neurosci. 2015;35:13827–35. Article CAS PubMed PubMed Central Google Scholar Izquierdo P, Attwell D, Madry C. Ion channels and receptors as determinants of microglial function. Trends Neurosci. 2019;...
Brain iron dysregulation associated with aging is closely related to motor and cognitive impairments in neurodegenerative diseases. The regulation of iron traffic at the blood-brain barrier (BBB) is crucial to maintain brain iron homeostasis. However, th
culture medium was changed every 2 days. These cultures were maintained for up to 12–14 days in vitro (DIV) to allow for the development and maturation of dendritic synapses and spines. This protocol produced a neuron-enriched culture and 90–95% of cells were co-stained with the NeuN ...
Changes in the Spontaneous Calcium Oscillations for the Development of the Preconditioning-Induced Ischemic Tolerance in Neuron/Astrocyte Co-culture Changes in the spontaneous calcium oscillations for the development of the preconditioning-induced ischemic tolerance in neuron/astrocyte co-culture[J].{H}.....
Studies in Drosophila showed that the involvement of astrocytes in the regulation of circadian rhythms5,6 was mediated by a clock neuron peptide transmitter, pigment dispersing factor6, which acts on a receptor similar to that for vasoactive intestinal polypeptide (VIP) in mammals. VIP is an ...