Previously, we reported that a cell population derived from human fetal bone marrow (BM), termed here Flk1 + CD34 postembryonic pluripotent stem cells (PPSCs) that have the characteristics of mesenchymal stem cells (MSCs), could differentiate into ectodermal, endodermal and mesodermal cell types...
Primordial germ cells have been shown to be capable of generating ESC-like pluripotent stem cells called embryonic germ cells (EGCs). Mouse EGCs show the fundamental characteristics of pluripotent cells; they can generate teratomas, and can contribute to chimeric mice after injection into a blastocyst...
Induced pluripotent stem cells (iPSCs) are somatic cell-derived reprogrammed cells that have equivalent characteristics to ESCs. Disease-specific iPSCs have started to be used for the investigation of and development of the treatment for several diseases. These cells have been intensively used for ...
Embryonic stem cells (ESCs) are pluripotent stem cells that are derived from early embryos. Induced pluripotent stem cells (iPSCs) are somatic cell-derived reprogrammed cells that have equivalent characteristics to ESCs. Disease-specific iPSCs have started to be used for the investigation of and de...
The differentiation of the cells was induced using bFGF and feeder cell removal approach under serum-free conditions. The pigmentation and RPE morphology of the cells were analysed and the expression of genes and proteins characteristics for RPE cells were studied. The in vitro functionality of the...
Cell lines are extensively analyzed for purity and stem-cell characteristics, including recovery after thawing, absence of mycoplasma and bacteria, expression of stem cell-specific markers (OCT-4, NANOG, SSEA-3, SSEA-4, TRA-1-60, TRA-1-81), absence of differentiation markers (Beta-3 tubulin...
Although iPSCs resemble primary ESCs in terms of their cellular characteristics and the potential for differentiation into all lineages, limitations associated with reprogramming and persistent features of somatic cell identity render iPSCs distinct. Reprogramming of various somatic cell types reveals persiste...
Here, we use a porcine MI model to study EAs after phenotyping the composition of PSC-CM cell doses. We aimed to identify cellular characteristics predictive of arrhythmogenesis, hypothesizing that this would inform the safe production of CMs for future clinical use. We also sought to test ...
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Embryonic pluripotency can be recapitulated in vitro by a spectrum of pluripotent stem cell states stabilized with different culture conditions. Their distinct spatiotemporal characteristics provide an unprecedented tool for the study of early human development. The newly unveiled ability of some stem cell...