in E. coli. An approximate 1. 6 kb PvuII-Pst1 fragment or a 2.0 kb PvulI-Bal1 fragment of pBR322 contains the origin of replication. The sequence of pBR322 is provided in Sutcliffe, 1979. Cold Spring Harbor Symp. 43, 77-90. The pUC plasmids, containing the lac promoter, contain ...
be useful for cloning genes that may be deleterious at high copy number. Since the pCL1920/21 vectors are compatible with ColE1-derived plasmids they can be used to form stable co-transformants together with pBR322 or pUC derived plasmids (Lerner et al.,Nucleic Acids Res.,18:4631 (1990)...
50 µg of pBR322 and 25 µg of lambda phage DNA b. 2 .56 g CsCl (ρ: 1.57 g/mL) in 2.55 mL TE buffer (pH 8.0) with 33 µL EtBr (10 mg/mL) 2. F ill centrifuge tubes to capacity and overlay with supplementary liquid [CsCl (1 g) dissolved in TE buffer (1 mL)...
This has been shown mainly by transduction of λoriR1 into recipients containing R1 plasmids or plasmid pBR322 carrying the basic replicon. We were able to demonstrate that a copy mutant of plasmid R1 was insensitive to copA +, but sensitive to the conserted action of Pst1 fragments F1 and...