Bolivar and co-workers engineeredpBR322as acloning vectorseveral years back (Bolívar et al., 1977a,b). One of its most outstanding features is that it contains the uni-directionalreplication origin ofthe naturalColE1plasmid. As a consequence, the 2D gel patterns generated by the RIs of pBR...
The fate of recombinant DNA in natural environments probably depends primarily on the nature of its bacterial host and the cloning vector in which it is carried. To minimize the potential transfer of plasmids containing novel genes from a released GEMMO to indigenous bacteria, the novel genes are...
Natural antioxidantDNA damagePlasmid DNANaturally occurring compounds capable of protecting DNA against ionizing radiation and chemical mutagens have considerable potential for prevention of mutation-based health impairment including cancer and other degenerative diseases. Chlorophyllin (CHL), a water-soluble ...