Bolivar and co-workers engineeredpBR322as acloning vectorseveral years back (Bolívar et al., 1977a,b). One of its most outstanding features is that it contains the uni-directionalreplication origin ofthe naturalColE1plasmid. As a consequence, the 2D gel patterns generated by the RIs of pBR...
(pbr322 and puc19). artificial vectors have a better replication rate than natural cloning vectors. q3 what is the abbreviation of ‘pbr322’? the ‘p’ denotes it is a plasmid cloning vector. the ‘br’ stands for boliver and rodriguez. finally, the number 322 segregates this vector from...
This chapter discusses pBR322, which is is a 4,362-bp double-stranded DNA plasmid cloning vehicle designed to allow simple and rapid preparation of cloned recombinant DNA fragments. It contains two antibiotic resistance genes, an origin of replication, and a variety of useful restriction sites for...
Medical School of Nanjing University,Nanjing 210093) Abstract: The specific aim of this work is to probe the formation process of DNA with cruciform extrusion in a pBR322 plasmid using spectroscopic methods while without digesting DNA into pieces. Pyrrolo deoxycitidine(PdC)was incorporated into ...
Molecular recognition between oligopeptides and nucleic acids: DNA binding selectivity of a series of 1,2,4-triazole-containing lexitropsins The synthesis of a series of 1,2,4-triazole-containing oligopeptide lexitropsins related to the natural antitumor antibiotic distamycin is described. The b......
Natural antioxidantDNA damagePlasmid DNANaturally occurring compounds capable of protecting DNA against ionizing radiation and chemical mutagens have considerable potential for prevention of mutation-based health impairment including cancer and other degenerative diseases. Chlorophyllin (CHL), a water-soluble ...