recombination PTO 35''** PTO 5'** P 3' kan ssLD OR kan ssLG 3' P 3' 3' ** 5' PTO **3' 5' PTO Amp pBR322 Ori pBAD vector 5.29 Kb KMS KMR f 600 Beta recombination ssLD ssLG 400 200 0 Figure 1. Redβ requires both N- and C-terminae for homologous recombination....
That target plasmid single or low the plasmid of the number of copies, for example is the BAC vector, that is useful in cloning of big DNA fragment. For example as for that transposon plasmid, the landlord bacterium the colon bacillus while conforming or it can include the duplication ...
is attached to Cla I site of pBR 322. This recombinant vector is used to transition Escherichia coli. The cells subjected to transformation are plated on two different media, one containing ampicillin and the other containing tetracycline. The transformed cells containing the recombinant vector will ...
The sequence in italics contains an EcoRI site which was used for the subsequent cloning step. These products were ligated into pGEM-T Easy and subsequently directionally cloned into CHAB using the engineered EcoRI and XhoI sites. Note that all of the constructs tested were cloned into CHAB ...
One of two target replicons was also present: these carried a pBR322 origin of replication, the tus gene under control of para, the araC repressor gene, a Ter sequence in the non-permissive orientation (Ternp, pBS172) or in the permissive orientation (Terp, pBS173) in front of that a...
It is possible to introduce “intercalating” sequences-between one or other of the flanking sequences and the foreign gene, for example sequences making possible the selection of the transformants, markers, sequences making possible the cloning of the vector, etc . . . ...
Useful expression vectors for bacterial use can comprise a selectable marker and bacterial origin of replication derived from commercially available plasmids comprising genetic elements of the well known cloning vector pBR322 (ATCC 37017). Such commercial vectors include, for example, pKK223-3 (...
lanuginosus DNA was prepared by using the X Charon 3A vector (12, 14). In addition, to isolate the intact repeating rDNA unit, a library in X Charon 4A was made by partially digesting genomic DNA with EcoRI restriction endonuclease (digestion conditions, 0.5 unit of enzyme per ,g of ...
(PT3167-1)”,Clonetech Laboratories GmbH, Heidelberg, Germany, 1997 I.B.R.). The plasmid DNA was then cleaved with the restriction enzyme HindIII (Pharmacia Biotech Europe GmbH, Freiburg, Germany) and ligated in theEscherichia colicloning vector pK18mob2 (Tauch et al., Plasmid 40, 126-...
2.A method for eliciting or enhancing an immune response to HER-2/neu protein, comprising infecting antigen presenting cells of a warm-blooded animal ex vivo with a viral vector and subsequently delivering the infected cells to the animal in an amount effective to elicit or enhance said response...