The EcoRI restriction fragment of wild-type Escherichia coli DNA that can complement the argG mutation was cloned into the EcoRI site on pBR322. The resulting chimeric plasmid (pYN81) contains a 16.0 kilobase fragment and can complement the nusA1 (Friedman 1971) as well as argG mutations. ...
The plasmid pBR322 had been observed to be excluded from bacteria also containing pUC19. To investigate whether plasmid size and the presence of the rop gene are involved in the exclusion of pBR322 by its successor pUC19, removal of a 1.1kb fragment from the pBR322 vector was attempted to ...
A 1.8 kb DNA fragment, liberated by endonuclease HindIII, contains the control region of the argECBH bipolar operon near one end and the weak secondary promoter of argH at the other extremity; it has been cloned in plasmid pBR322. The same plasmid vector has been used to clone the argF ...
The method depends on circular integration of the vector into the chromosome of a host nonpermissive for its replication, and on excision and reduction of a recombinant plasmid by use of an appropriately designed set of restriction enzyme sites in the vector. A vector suitable for cloning in ...
In order to delimit the approximate boundaries of the marmoset herpesvirus (MarHV) thymidine kinase (TK) gene, HindIII and BamHI digests of MarHV DNA were cloned in plasmid pBR322. Several recombinant plasmids which transformed E. coli K12 strain RR1 to ampicillin resistance were isolated. The...
In order to clone the 462 bp Alu I fragment containing the lpp promoter region in pBR322, the DNA fragment lying between the Eco RI and Hind III cleavage sites of pBR322 (containing the promoter of the tetracycline resistance gene) was first deleted, as shown schematically at 106 in FIG....
Hence, in the presence of the LacIq repressor, IPTG must be provided to inhibit the LacIq. Inhibition of LacIq permits expression from the lac promoter for blue/white screening. 何为ori? ori是复制起点,即复制起始处的序列。它决定了载体拷贝数...
III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generate... In vitro recombinant DNA techniques were used to construct two new cloning vehicles, pBR324 and pBR235. These vectors, derived from plasmid pBR322, are rel... Francisco,Bolivar - 《Gene》 ...
. pBR322 has been constructed in vitro from the tetracycline resistance gene (Tc) from pSC101, the origin of DNA replicon (ori) from the colE1 derivative pMB1, and the ampicillin resistance gene (Amp) from transposon Tn3. The ori from pBR322 allows replication of plasmid pYAC2 in E. ...
For propagation and selection in Escherichia coli, the plasmids contain the pBR322 origin of replication and the beta-lactamase-encoding gene. Within a set, each of the three plasmids carries the unique SmaI and BamHI restriction sites in a different reading frame relative to the 'lacZ gene. ...