pBS(+) or pBluescript (Statagene) with promoters for T3 and T7 RNA polymerase flanking the multiple cloning site Escherichia coli strains MC1061 or DH5α (Life Technologies) or SURE (Stratagene) View chapterExplore book DNA Repair Enzymes: Cell, Molecular, and Chemical Biology Youngho Kwon,...
pBluescript II SK (+/–) Multiple Cloning Site Region (sequence shown 598–826)Sac I BstX I ...GGTATCGATAAGCTTGATATCGAATTCCTGCAGCCCGGGGGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTC...Sac II TTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAGGTCGAC... Acc I Xho I...
pBluescript II Phagemid Vectors 3 pBluescript II KS (+/–) Phagemids f1 (+) ori f1 (-) ori ampicillin pBluescript II KS (+/-) 3.0 kb lacZ' Sac I MCS Kpn I P lac pUC ori pBluescript II KS (+/–) Multiple Cloning Site Region (sequence shown 598–826) BssH II T7 Promoter...
pbluescriptprimerbsshbindingsitekpn f1(+)ori MCS lacZ' Plac ampicillin pUCori KpnI SacI pBluescriptIISK(+) 3.0kb pBluescriptIISK(+/–)MultipleCloningSiteRegion (sequenceshown598–826) SacIBstXI ...GGTATCGATAAGCTTGATATCGAATTCCTGCAGCCCGGGGGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTC... EcoRIEcoR...
pBluescript II XR cDNA Library Construction Kit pBluescript II SK (+) Vector Map f1 (+) ori ampicillin pBluescript II SK (+) 3.0 kb lacZ' Kpn I MCS Sac I P lac pUC ori pBluescript II SK (+) Multiple Cloning Site Region (sequence shown 598–826) BssH II T7 Promoter Kpn I ...
one of multiple similar variants" promoter 626..644 /label="T7 promoter" /label="promoter for bacteriophage T7 RNA polymerase" misc_feature 653..760 /label="MCS" /label="pBluescript multiple cloning site" primer_bind 670..686 /label="KS primer" /label="common sequencing primer, one of...
pBluescript_SK_plus质粒pSK图谱
multiple similar variants" promoter 626..644 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 653..760 /note="MCS" /note="pBluescript multiple cloning site" primer_bind 670..686 /note="KS primer" /note="common sequencing primer, one of multiple ...