pBR322是一个人工构建的重要质粒，有万能质粒之称。它是由pSF2124、pMB8及pSC101三个亲本质粒经复杂的重组过程构建而成的。优点 1质粒载体pBR322的大小为4361bp，相对分子质量较小 2它带有一个复制起始位点，保证了该质粒只在大肠杆菌的细胞中行使复制的功能。3具有两种抗生素抗性基因——氨苄青霉素抗性...
The plasmid pE10 is a pBR322-derived plasmid carrying a 4.5 kb rice (Oryza sativa L.) repeated DNA sequence. The cloned sequence has been shown to be amplified in cultured rice cells. The analysis of practically intact chromosomal rice DNA molecules by pulsed field gel electrophoresis has now...
What is the role of reverse transcriptase in a retrovirus infection? What is plasmid transformation? What is the role of RNA polymerase? What is the role of RNA polymerase in protein synthesis? What role does DNA helicase play in replication?
A complete, cloned complementary DNA copy of the RNA genome of poliovirus was constructed in the Pst I site of the bacterial plasmid pBR322. Cultured mammalian cells transfected with this hybrid plasmid produced infectious poliovirus. Cells transfected with a plasmid which lacked the first 115 bases...
A. 矛盾双方在一定条件下可以相互转化 B. 矛盾既具有普遍性又具有特殊性 C. 内因和外因相结合推动事物发展 D. 事物发展是量变和质变的统一 查看完整题目与答案 以下哪种载体最适于构建植物表达载体?( ) A. pBR322; B. pBluescript vector; C. Ti plasmid; D. Cosmids 查看完整题目与答案 ...
Although there is no significant advantage to using higher-copy-number plasmids over pBR322-based vectors in terms of protein production yields, a high-copy plasmid might be your first port of call if you experience low protein yields.
Identify the correct answer regarding pBR322. It is a 1) bacteriophage. 2) yeast. 3) plasmid. 4) parasite. Select the correct answer. A cell which defends against body cells in which viruses are reproducing is [{Blank}]. A. Exotoxin B. Cytotoxic T cell C. Endotoxin D. Suppress...
2A). We also performed DNA sequencing reactions upon the 5.4 kb plasmid using the same primer and electrophoresed these reactions alongside the primer extension products. A primer extension product was generated only in the 15–17 h collection lane, and migrated with the thymine at position 101...
A study of mechanisms of electrotransfection using Escherichia coli (JM 105) and the plasmid DNA pBR322 as model system is reported. pBR322 DNA carries an ... Xie, T.D.,L Sun,Tsong, T.Y. - 《Biophysical Journal》 被引量: 310发表: 1990年 Prediction of Charge-Induced Molecular Alignment...
Plasmid pBR322.HPV31 has been previously described (Stubenrauch et al., 1998b). Single and double mutations of splice signals (pBR322-HPV31-SD1426mt, pBR322-HPV31-SD1534mt, pBR322-HPV31-SA3332mt, pBR322-HPV31-SD1426/SD1534mt, pBR322-HPV31-SD1426/SD1534/SA3332mt) were introduced ...